Suzuki Nobuo, Hattori Atsuhiko
Noto Marine Laboratory, Institute of Nature and Environmental Technology, Kanazawa University, Uchiura, Ishikawa 927-0553, Japan.
Life Sci. 2003 Sep 12;73(17):2237-47. doi: 10.1016/s0024-3205(03)00603-9.
The direct effect of bisphenol A on osteoclasts and osteoblasts was examined using a culture system of goldfish scales. Tartrate-resistant acid phosphatase (TRAP) and alkaline phosphatase (ALP) were used as markers of osteoclasts and osteoblasts, respectively. Bisphenol A (10(5) M) significantly suppressed both TRAP and ALP activities. These data were reproduced in an in vivo experiment. From an analysis of a reverse transcription-polymerase chain reaction in the in vitro-cultured scales, it was demonstrated that the insulin-like growth factor (IGF)-1 mRNA expression decreased by a bisphenol A treatment. On the other hand, 17beta-estradiol (E(2)) stimulated both TRAP and ALP activities and did not change IGF-1 mRNA expression, suggesting that bisphenol A has a different effect from E(2) on bone metabolism. This study is the first to demonstrate that bisphenol A functions to suppress directly osteoblasts and osteoclasts among vertebrates, which strongly suggests that this scale in an in vitro assay system can be utilized for the evaluation of the effects of endocrine disrupters on bone cells.
利用金鱼鳞片培养系统研究了双酚A对破骨细胞和成骨细胞的直接作用。分别使用抗酒石酸酸性磷酸酶（TRAP）和碱性磷酸酶（ALP）作为破骨细胞和成骨细胞的标志物。双酚A（10⁵M）显著抑制了TRAP和ALP的活性。这些数据在体内实验中得到了重现。通过对体外培养鳞片中的逆转录聚合酶链反应分析表明，双酚A处理可降低胰岛素样生长因子（IGF）-1 mRNA的表达。另一方面，17β-雌二醇（E₂）刺激了TRAP和ALP的活性，并且没有改变IGF-1 mRNA的表达，这表明双酚A在骨代谢方面与E₂具有不同的作用。本研究首次证明双酚A在脊椎动物中直接发挥抑制成骨细胞和破骨细胞的作用，这有力地表明体外分析系统中的这种鳞片可用于评估内分泌干扰物对骨细胞的影响。
