Centre for Forensic Science, University of Strathclyde, Glasgow G1 1XW, UK.
Int J Legal Med. 2012 Jan;126(1):161-5. doi: 10.1007/s00414-011-0558-5. Epub 2011 Feb 19.
Here, we applied the Taguchi method, an engineering optimization process, to successfully determine the optimal conditions for three SYBR Green I-based quantitative PCR assays. This method balanced the effects of all factors and their associated levels by using an orthogonal array rather than a factorial array. Instead of running 27 experiments with the conventional factorial method, the Taguchi method achieved the same optimal conditions using only nine experiments, saving valuable resources.
在这里，我们应用田口方法（一种工程优化过程）成功确定了三种基于 SYBR Green I 的实时定量 PCR 检测的最佳条件。该方法通过使用正交数组而不是析因数组来平衡所有因素及其相关水平的影响。与传统析因法需要进行 27 次实验相比，田口方法仅使用 9 次实验就达到了相同的最佳条件，从而节省了宝贵的资源。
