Tange T, Shimizu K, Imashiro S, Kumasaka S, Higaki M
Department of Pedodontics, Kanagawa Dental College.
Shoni Shikagaku Zasshi. 1990;28(2):359-70.
Trace elements (Cd, Zn, Pb and Cu) levels were determined in lower deciduous incisors which were collected from four areas (Sapporo, Yokosuka, Nagano and Kitakyushu) in Japan. Samples were divided into enamel and dentin, and dissolved with nitric and dried at 80 degrees C. Each element was separated chromatographically using an acetone-HCL gradient solution with a cation exchange resin and then analyzed by atomic absorption spectrophotometry. The results were as follows: 1. Ca, P, Ca/P ratio levels showed no significant differences among four areas. 2. In the enamel, (1) Cd levels of each area were 0.593 micrograms/g (Yokosuka) greater than 0.315 micrograms/g (Sapporo) greater than 0.261 micrograms/g (Nagano) greater than 0.222 micrograms/g (Kitakyushu). (2) Zn levels of each area were 174.912 micrograms/g (Nagano) greater than 157.473 micrograms/g (Yokosuka) greater than 150.971 micrograms/g (Kitakyushu) greater than 128.980 micrograms/g (Sapporo). (3) Pb levels of each area were 8.412 micrograms/g (Kitakyushu) greater than 7.925 micrograms/g (Yokosuka) greater than 5.426 micrograms/g (Sapporo) greater than 4.285 micrograms/g (Nagano). (4) Cu levels of each area were 9.276 micrograms/g (Kitakyushu) greater than 6.928 micrograms/g (Nagano) greater than 6.178 micrograms/g (Yokosuka) greater than 4.389 micrograms/g (Sapporo). 3. In the dentin, (1) Cd levels of each area were 0.214 micrograms/g (Sapporo) greater than 0.184 micrograms/g (Kitakyushu) greater than 0.145 micrograms/g (Nagano) greater than 0.119 micrograms/g (Yokosuka). (2) Zn levels of each area were 124.152 micrograms/g (Yokosuka) greater than 112.242 micrograms/g (Nagano) greater than 111.952 micrograms/g (Sapporo) greater than 111.456 micrograms/g (Kitakyushu). (3) Pb levels of each area were 3.584 micrograms/g (Kitakyushu) greater than 3.019 micrograms/g (Yokosuka) greater than 1.819 micrograms/g (Sapporo) greater than 1.523 micrograms/g (Nagano). (4) Cu levels of each area were 3.734 micrograms/g (Kitakyushu) greater than 2.221 micrograms/g (Nagano) greater than 2.098 micrograms/g (Yokosuka) greater than 1.664 micrograms/g (Sapporo). Therefore, the results suggest that the trace elements levels in deciduous teeth depended on the environmental condition of each area and the deciduous teeth could be a useful material as an environmental contamination indicator.
对从日本四个地区（札幌、横须贺、长野和北九州）采集的下颌乳切牙中的微量元素（镉、锌、铅和铜）水平进行了测定。样本被分为牙釉质和牙本质，用硝酸溶解并在80摄氏度下干燥。每种元素用丙酮 - 盐酸梯度溶液和阳离子交换树脂进行色谱分离，然后用原子吸收分光光度法进行分析。结果如下：1. 钙、磷、钙/磷比值在四个地区之间无显著差异。2. 在牙釉质中，（1）各地区的镉含量为横须贺（0.593微克/克）＞札幌（0.315微克/克）＞长野（0.261微克/克）＞北九州（0.222微克/克）。（2）各地区的锌含量为长野（174.912微克/克）＞横须贺（157.473微克/克）＞北九州（150.971微克/克）＞札幌（128.980微克/克）。（3）各地区的铅含量为北九州（8.412微克/克）＞横须贺（7.925微克/克）＞札幌（5.426微克/克）＞长野（4.285微克/克）。（4）各地区的铜含量为北九州（9.276微克/克）＞长野（6.928微克/克）＞横须贺（6.178微克/克）＞札幌（4.389微克/克）。3. 在牙本质中，（1）各地区的镉含量为札幌（0.214微克/克）＞北九州（0.184微克/克）＞长野（0.145微克/克）＞横须贺（0.119微克/克）。（2）各地区的锌含量为横须贺（124.152微克/克）＞长野（112.242微克/克）＞札幌（111.952微克/克）＞北九州（111.456微克/克）。（3）各地区的铅含量为北九州（3.584微克/克）＞横须贺（3.019微克/克）＞札幌（1.819微克/克）＞长野（1.523微克/克）。（4）各地区的铜含量为北九州（3.734微克/克）＞长野（2.221微克/克）＞横须贺（2.098微克/克）＞札幌（1.664微克/克）。因此，结果表明乳牙中的微量元素水平取决于各地区的环境状况，乳牙可能是一种有用的环境污染指示材料。
