Nantia Edouard Akono, Travert Carine, Manfo Faustin-Pascal T, Carreau Serge, Monsees Thomas K, Moundipa Paul Fewou
Laboratory of Pharmacology and Toxicology, Department of Biochemistry, University of Yaoundé I, PO. Box 812 Yaounde, Cameroon; E-Mails:
Int J Mol Sci. 2011 Jan 14;12(1):376-84. doi: 10.3390/ijms12010376.
In this study, Leydig cells were purified from 70 day-old Sprague Dawley male rats and incubated with 10 and 100 μg/mL of methanol extract of Basella alba (MEBa) for 4 hours followed by the evaluation of cell viability, steroid (testosterone and estradiol) production, and the level of aromatase mRNA. Results showed that MEBa did not affect Leydig cell viability. At the concentration of 10 μg/mL, MEBa significantly stimulated testosterone and estradiol production (p < 0.01 and p < 0.03, respectively), and enhanced aromatase mRNA level (p < 0.04). These observations suggest that MEBa directly stimulated testosterone, estradiol and aromatase mRNA levels in isolated Leydig cells.
在本研究中，从70日龄的斯普拉格-道利雄性大鼠中纯化出睾丸间质细胞，并用10和100μg/mL的落葵甲醇提取物（MEBa）孵育4小时，随后评估细胞活力、类固醇（睾酮和雌二醇）生成以及芳香化酶mRNA水平。结果显示，MEBa不影响睾丸间质细胞活力。在10μg/mL的浓度下，MEBa显著刺激睾酮和雌二醇生成（分别为p < 0.01和p < 0.03），并提高芳香化酶mRNA水平（p < 0.04）。这些观察结果表明，MEBa直接刺激分离的睾丸间质细胞中睾酮、雌二醇和芳香化酶mRNA水平。
