大花芙蓉葵与落葵混合物提取物对成年大鼠睾丸切片体外睾酮生成的影响。

Effects of extracts from Hibiscus macranthus and Basella alba mixture on testosterone production in vitro in adult rat testes slices.

作者信息

Moundipa Paul F, Ngouela Silvère, Kamtchouing Pierre, Tsamo Etienne, Tchouanguep Félicité M, Carreau Serge

机构信息

Laboratory of Nutrition and Biochemical Toxicology, University of Yaoundé I, P. O. Box 812, Yaoundé, Cameroon.

出版信息

Asian J Androl. 2006 Jan;8(1):111-4. doi: 10.1111/j.1745-7262.2006.00057.x.

DOI:10.1111/j.1745-7262.2006.00057.x

PMID:16372129

Abstract

AIM

To find an in vitro system for the measurement of the androgenic effects of different extracts of Hibiscus macranthus (Malvaceae) and Basella alba (Basellaceae).

METHODS

The production of testosterone from testes slices incubated in two media, either Krebs-Henseleit buffer containing 0.5% Bovine serum albumin (BSA) or Dubecco's Modified Eagle's medium-F12 Ham nutrient mixture (DME/Ham F12), under a mixture of 5% CO2 in 95% air was determined either in the presence or absence of cofactors and Hibiscus macranthus plus Basella alba (HMBA) extracts.

RESULTS

The testosterone production was increased in testes slices incubated in DME/Ham F12 medium in response to the cofactors (49%) and aqueous extracts (34%-60% according to dilutions). Under the same atmospheric conditions, there was no positive response of the testes slices to either cofactor or HMBA extract stimulation in Krebs-Henseleit buffer containing 0.5% BSA. In further investigations related to the effect of HMBA, the DME/Ham F12 medium was used. The results obtained from the in vitro test showed that the activity was present mainly in methylene chloride and methanol, since these extracts induced an increase in testosterone production by testes slices.

CONCLUSION

The testes slice system is suitable to be used for further in vitro investigations of the isolation of androgenic bioactive components of plants.

摘要

目的

寻找一种体外系统，用于测量大花芙蓉（锦葵科）和落葵（落葵科）不同提取物的雄激素效应。

方法

在含有5%二氧化碳和95%空气的混合气体环境下，将睾丸切片置于两种培养基中孵育，一种是含有0.5%牛血清白蛋白（BSA）的Krebs-Henseleit缓冲液，另一种是杜氏改良伊格尔培养基-F12哈姆营养混合物（DME/Ham F12），分别在有无辅因子以及大花芙蓉加落葵（HMBA）提取物的情况下，测定睾酮的生成量。

结果

在DME/Ham F12培养基中孵育的睾丸切片，对辅因子（49%）和水提取物（根据稀释度不同，增加34%-60%）有反应，睾酮生成量增加。在相同的大气条件下，在含有0.5% BSA的Krebs-Henseleit缓冲液中，睾丸切片对辅因子或HMBA提取物刺激均无阳性反应。在与HMBA作用相关的进一步研究中，使用了DME/Ham F12培养基。体外试验结果表明，活性主要存在于二氯甲烷和甲醇提取物中，因为这些提取物能使睾丸切片的睾酮生成量增加。