利用负选择标记sacB进行基因替换和转座子递送
Gene Replacement and Transposon Delivery Using the Negative Selection Marker sacB.
作者信息
Jackson M, Reinaldo Camacho L, Gicquel B, Guilhot C
出版信息
Methods Mol Med. 2001;54:59-75. doi: 10.1385/1-59259-147-7:059.
PMID:21341069
Abstract
Gene replacement and transposon mutagenesis are two complementary tools that have been widely used to perform genetic studies in various living organisms. In mycobacteria, and especially in the Mycobacterium tuberculosis complex, the lack of these tools has severely hampered the genetic studies of these organisms, especially regarding the identification and characterization of virulence genes of these important pathogens.
摘要
基因替换和转座子诱变是两种互补的工具，已被广泛用于在各种生物体中进行遗传学研究。在分枝杆菌中，尤其是在结核分枝杆菌复合群中，缺乏这些工具严重阻碍了对这些生物体的遗传学研究，特别是在鉴定和表征这些重要病原体的毒力基因方面。
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