Institute of Traditional Medicine, National Yang-Ming University, Taipei, Taiwan.
Planta Med. 2011 Sep;77(13):1495-503. doi: 10.1055/s-0030-1270783. Epub 2011 Feb 21.
Platelet-derived growth factor (PDGF) induces cell proliferation together with oxidative stress. The present study investigated the effects of salvianolic acid A (Sal A) and B (Sal B) on the PDGF-induced signaling cascades in hepatic stellate cells (HSCs). HSC-T6, a rat hepatic stellate cell line, was stimulated with PDGF (10 ng/mL). The inhibitory effects of Sal A and B on oxidative stress-related signaling pathways were assessed in vitro. The protein levels were measured by Western blotting. FACS analysis was applied to detect the thioredoxin (Trx) level. Sal A and B showed different inhibitory abilities on the PDGF-related pathway. Sal A inhibited 70-kDa ribosomal S6 kinase (p70(s6k)) and associated proteins. Sal B attenuated PDGF-induced c-jun-N-terminal kinase (JNK), p38, and PKC- δ phosphorylations. Both Sal A and B diminished the activation of PKD, Trx, heme-oxygenase (HO)-1, and Nrf2. Taken together, our results showed that Sal A and B attenuated PDGF-induced ROS formation in HSCs, possibly through different signaling pathways.
血小板衍生生长因子 (PDGF) 可诱导细胞增殖和氧化应激。本研究探讨了丹酚酸 A（Sal A）和 B（Sal B）对肝星状细胞（HSCs）中 PDGF 诱导的信号级联的影响。PDGF（10ng/mL）刺激大鼠肝星状细胞系 HSC-T6。体外评估 Sal A 和 B 对氧化应激相关信号通路的抑制作用。通过 Western blot 测定蛋白水平。应用 FACS 分析检测硫氧还蛋白（Trx）水平。Sal A 和 B 对 PDGF 相关途径表现出不同的抑制能力。Sal A 抑制 70kDa 核糖体 S6 激酶（p70(s6k)) 和相关蛋白。Sal B 减弱了 PDGF 诱导的 c-jun-N-末端激酶 (JNK)、p38 和 PKC-δ磷酸化。Sal A 和 B 均减弱了 PKD、Trx、血红素加氧酶 (HO)-1 和 Nrf2 的激活。总之，我们的结果表明 Sal A 和 B 可减轻 HSCs 中 PDGF 诱导的 ROS 形成，可能通过不同的信号通路。
