Ningbo Key Laboratory of Poison Research and Control, Ningbo Municipal Center for Disease Control and Prevention, Ningbo 315010, People's Republic of China.
Anal Biochem. 2011 Jun 15;413(2):142-7. doi: 10.1016/j.ab.2011.02.020. Epub 2011 Feb 20.
Chlorophacinone (CPN) is an extensively used anticoagulant rodenticide in China. A simple, rapid, and sensitive method is necessary for the determination of CPN in clinical emergency rescue. In this study, a novel fluorimetric method for the determination of CPN in human serum was developed. This proposed method is based on luminescence produced by a formation of a ternary CPN-europium-dl-histidine complex system. The fluorescence intensity was enhanced by adding yttrium and polyvinylpyrrolidone colloid-type suspension at pH 9.0. All of the important methodological achievements, such as working wavelengths, pH for optimal yttrium fluorescence enhancement, and the method recovery, limit of detection, limit of quantification, and relative standard deviation together with its linearity range, are discussed in detail. The developed method was successful in the determination of CPN in actual human serum in both clinical and forensic specimens. In comparison with the liquid chromatography tandem mass spectrometry method, this method is fast, simple, and less expensive.
氯鼠酮（CPN）是中国广泛使用的抗凝血灭鼠剂。在临床急救中，需要一种简单、快速、灵敏的方法来测定 CPN。本研究建立了一种测定人血清中 CPN 的新型荧光法。该方法基于形成三元 CPN-铕-dl-组氨酸配合物体系产生的荧光。在 pH 9.0 时，通过加入钇和聚乙烯吡咯烷酮胶体悬浮液，增强了荧光强度。详细讨论了工作波长、最佳钇荧光增强 pH 值以及方法回收率、检测限、定量限和相对标准偏差及其线性范围等重要方法学成就。该方法成功地应用于临床和法医标本中实际人血清中 CPN 的测定。与液相色谱串联质谱法相比，该方法快速、简单、廉价。
