Quesada A, Prada F A, Espinar A, Génis-Gálvez J M
Developmental Biology Institute, Faculty of Medicine, Sevilla, Spain.
Histol Histopathol. 1990 Jul;5(3):315-24.
The effect of ethanol on the cerebellar cortex of chick embryos was studied in semi-thin sections of material prepared for electron microscopy. The embryos were injected with ethanol on the 3rd or 6th day of incubation and observed until days 13, 15, 17 and 21 of development. A decrease was seen in the number of germinal cells generated, together with defects in neuronal migration and the existence of a lower quantity of cells due to a generalised process of cell death. At the same time, a progressive neuronal degeneration was observed until the 15th day of incubation, the tissue recovering progressively on days 17 and 21. On the other hand, the embryos treated with ethanol on the 3rd day were less affected than those injected on the 6th day.
通过用于电子显微镜检查的材料半薄切片，研究了乙醇对鸡胚小脑皮质的影响。在孵化的第3天或第6天给胚胎注射乙醇，并观察至发育的第13、15、17和21天。结果发现，生发细胞数量减少，同时存在神经元迁移缺陷以及由于普遍的细胞死亡过程导致细胞数量减少。与此同时，在孵化第15天之前观察到神经元进行性退化，在第17天和第21天组织逐渐恢复。另一方面，在第3天用乙醇处理的胚胎比在第6天注射的胚胎受影响小。
