Department of Chemistry, Tarbiat Modares University, Tehran, Iran.
J Sep Sci. 2011 Mar;34(5):585-93. doi: 10.1002/jssc.201000735. Epub 2011 Jan 18.
Electromembrane extraction coupled with high-performance liquid chromatography (HPLC) and ultraviolet (UV) detection was developed for the determination of levamisole in some human biological fluids. Levamisole migrated from 4 mL of different acidized biological matrices, through a thin layer of 2-nitrophenyl octyl ether containing 5% tris-(2-ethylhexyl) phosphate immobilized in the pores of a porous hollow fiber, into a 20-μL acidic aqueous acceptor solution present inside the lumen of the fiber. The parameters influencing electromigration were investigated and optimized. Within 15 min of operation at 200 V, levamisole was extracted from different biological fluid samples with recoveries in the range of 59-65%, which corresponded to preconcentration factors in the range of 118-130. The calibration curves showed linearity in the range of 0.5-10, 0.2-10 and 0.1-10 μg/mL for plasma, urine and saliva, respectively. Limits of detection of 0.1, 0.07 and 0.05 μg/mL and limits of quantification of 0.5, 0.2 and 0.1 μg/mL were obtained for plasma, urine and saliva, respectively. The relative standard deviations of the analysis were found to be in the range of 5.6-9.7% (n = 3). Electromembrane extraction was successfully processed for determination of levamisole in plasma, urine and saliva samples.
采用高效液相色谱（HPLC）和紫外（UV）检测，结合电膜萃取技术，开发了一种测定人生物体液中左旋咪唑的方法。左旋咪唑从 4 毫升不同酸化的生物基质中，通过一层含有 5%三（2-乙基己基）磷酸酯的 2-硝基苯基辛基醚，迁移到纤维内腔中的 20μL 酸性接受溶液中，该纤维的孔中固定有 2-硝基苯基辛基醚。考察并优化了影响电动迁移的参数。在 200 V 下运行 15 min 内，从不同生物流体样品中提取左旋咪唑，回收率在 59-65%范围内，对应的预浓缩因子在 118-130 范围内。校准曲线在血浆、尿液和唾液中的线性范围分别为 0.5-10、0.2-10 和 0.1-10 μg/mL。血浆、尿液和唾液的检测限分别为 0.1、0.07 和 0.05 μg/mL，定量限分别为 0.5、0.2 和 0.1 μg/mL。分析的相对标准偏差在 5.6-9.7%（n=3）范围内。成功地对血浆、尿液和唾液样品中的左旋咪唑进行了电膜萃取分析。
