Yao Ping-Ping, Zhu Han-Ping, Deng Xiao-Zhao, Xu Fang, Xie Rong-Hui, Yao Chen-Hui, Weng Jing-Qing, Zhang Yun, Yang Zhan-Qiu, Zhu Zhi-Yong
Zhejiang Center for Disease Prevention and Control, Hangzhou 310051, China.
Bing Du Xue Bao. 2010 Nov;26(6):465-70.
In order to analyze the molecular epidemiology of Hantavirus (HV) in Zhejiang Province, the complete M and S genome sequences of 12 HV strains from different hosts and locations in Zhejiang Province of China during the period of 1981-2007 were analyzed on genetic evolution by DNAstar and MEGA 4.0 software in this research. Phylogenetic analyses revealed that HTN and SEO strains were co-circulating in Zhejiang Province, and the difference in sequence similarity and the phylogeny was closely related to the isolated regions, but had no distinct relationship with the isolate year and the host, indicating a relationship between epidemiology of HFRS and the distribution region, especially in HTNV. The isolates in the same region could be assigned in same or near phylogenetic clade sharing high sequence similarity. Interestingly, the Gou3 strain and ZJ5 strain isolated from Jiande region in Zhejiang Province formed a distinct phylogenetic lineage in SEOV clade, and different from the other SEOV variants outside China. We believed that the special SEOV variants were distributed in Jiande region.
为分析浙江省汉坦病毒（HV）的分子流行病学特征，本研究运用DNAstar和MEGA 4.0软件，对1981 - 2007年期间从中国浙江省不同宿主和地点分离得到的12株HV的M和S基因全序列进行了遗传进化分析。系统发育分析显示，HTN株和SEOV株在浙江省共同流行，序列相似性和系统发育的差异与分离地区密切相关，但与分离年份和宿主无明显关系，这表明肾综合征出血热的流行病学与分布地区有关，尤其是汉坦病毒型（HTNV）。同一地区的分离株可归为同一或相近的系统发育分支，序列相似性高。有趣的是，从浙江省建德地区分离出的Gou3株和ZJ5株在SEOV分支中形成了一个独特的系统发育谱系，与中国境外的其他SEOV变异株不同。我们认为，这种特殊的SEOV变异株分布于建德地区。
