Département de chimie and Centre d'optique, photonique et laser (COPL), Université Laval, Québec (QC), Canada G1V 0A6.
ACS Nano. 2011 Mar 22;5(3):1888-96. doi: 10.1021/nn102776m. Epub 2011 Feb 23.
This study describes the preparation and characterization of a DNA sensing architecture combining the molecular recognition capabilities of a cationic conjugated polymer transducer with highly fluorescent core-shell nanoparticles (NPs). The very structure of the probe-labeled NPs and the polymer-induced formation of NP aggregates maximize the proximity between the polymer donor and acceptor NPs that is required for optimal resonant energy transfer. Each hybridization event is signaled by a potentially large number of excited reporters following the efficient plasmon-enhanced energy transfer between target-activated polymer transducer and fluorophores located in the self-assembled core-shell aggregates, resulting in direct molecular detection of target nucleic acids at femtomolar concentrations.
本研究描述了一种 DNA 传感结构的制备和特性，该结构结合了阳离子共轭聚合物传感器的分子识别能力和具有高荧光的核壳纳米粒子 (NPs)。探针标记的 NPs 的结构和聚合物诱导的 NP 聚集的形成最大限度地增加了聚合物供体和受体 NPs 之间的接近程度，这是实现最佳共振能量转移所必需的。每个杂交事件都由大量受激报告分子发出信号，这是由于目标激活的聚合物传感器和位于自组装核壳聚集体中的荧光团之间的等离子体增强能量转移效率高，导致在飞摩尔浓度下直接对目标核酸进行分子检测。
