Institute of Advanced Materials, Jiangsu Key Laboratory for Organic Electronics & Information Displays, and School of Materials Science and Engineering, Nanjing University of Posts & Telecommunications, 9 Wenyuan Road, Nanjing 210046, China.
Biosens Bioelectron. 2011 Jan 15;26(5):2154-64. doi: 10.1016/j.bios.2010.09.025. Epub 2010 Sep 22.
Conjugated polymers (CPs) with large, delocalised molecular structures exhibit unique optical and electrochemical characteristics that can be used as excellent sensing elements. Recently, research on chemical and biological sensors that use water-soluble CPs as transducers has generated intense interest. Two main sensing mechanisms are used for the detection of DNA-related events, such as hybridisation, mismatch, single nucleotide polymorphism (SNP), SNP genotyping, conformational changes, and cleavage of the nucleic acids. One mechanism takes advantage of the fluorescence resonance energy transfer (FRET) between CPs and a chromophore label on the nucleic acid probes in which a series of cationic polyfluorene, polythiophene and polyarylene derivatives are frequently used. The other mechanism relies on the conformational effects of CPs, which is induced by combination of the specific targets in which cationic polythiophene derivatives are often used. The electron transfer property of CPs are always used to design high sensitive electrochemical DNA biosensors. Here we review recent progress in the development of optical and electrochemical DNA biosensors based on water-soluble CPs.
具有大离域分子结构的共轭聚合物 (CPs) 表现出独特的光学和电化学特性，可作为优秀的传感元件。最近，使用水溶性 CPs 作为换能器的化学和生物传感器的研究引起了极大的兴趣。有两种主要的传感机制可用于检测与 DNA 相关的事件，例如杂交、错配、单核苷酸多态性 (SNP)、SNP 基因分型、构象变化和核酸的切割。一种机制利用 CPs 与核酸探针上的发色团标记之间的荧光共振能量转移 (FRET)，其中经常使用一系列阳离子聚芴、聚噻吩和聚芳基衍生物。另一种机制依赖于 CPs 的构象效应，这是由特定目标的组合引起的，其中经常使用阳离子聚噻吩衍生物。CPs 的电子转移性质通常用于设计高灵敏度的电化学 DNA 生物传感器。在这里，我们综述了基于水溶性 CPs 的光学和电化学 DNA 生物传感器的最新进展。
