Department of Histology, Microbiology and Medical Biotechnologies, University of Padova, via Gabelli 63, 35121 Padova, Italy.
Acta Biomater. 2011 Jun;7(6):2526-32. doi: 10.1016/j.actbio.2011.02.025. Epub 2011 Feb 21.
Structural, mechanical and biochemical properties have to be considered when searching for suitable extracellular matrix substitutes. Fibrous structures of synthetic or natural polymers have received increasing interest as three-dimensional scaffolds for tissue engineering applications as they can be easily produced by electrospinning with different topographical features by changing the process parameters. On the other hand, the nanobiotechnology approach suggests mimicking molecular architectures in nature through self-assembly. In particular, self-assembling peptide-based biomaterials have been successfully used as scaffolds for cell growth. In order to amalgamate these two strategies nanofibrous electrospun scaffolds of hybrid polymer were designed and obtained by mixing poly(ethylene oxide) and self-assembling peptides in aqueous solution. The results of in vitro osteoblast adhesion and proliferation assays on the electrospun scaffolds obtained using different self-assembling peptide sequences are discussed.
在寻找合适的细胞外基质替代品时，必须考虑结构、力学和生化特性。合成或天然聚合物的纤维结构作为组织工程应用的三维支架受到了越来越多的关注，因为它们可以通过改变工艺参数很容易地通过电纺丝来生产具有不同形貌特征的纤维。另一方面，纳米生物技术方法建议通过自组装来模拟自然界中的分子结构。特别是，基于自组装肽的生物材料已被成功用作细胞生长的支架。为了结合这两种策略，通过在水溶液中混合聚（氧化乙烯）和自组装肽来设计和获得混合聚合物的纳米纤维电纺支架。讨论了使用不同自组装肽序列获得的电纺支架上成骨细胞黏附和增殖试验的结果。
