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用于成骨细胞的电纺支架:肽诱导的聚己内酯浓度依赖性改善

Electrospun Scaffolds for Osteoblast Cells: Peptide-Induced Concentration-Dependent Improvements of Polycaprolactone.

作者信息

Dettin Monica, Zamuner Annj, Roso Martina, Gloria Antonio, Iucci Giovanna, Messina Grazia M L, D'Amora Ugo, Marletta Giovanni, Modesti Michele, Castagliuolo Ignazio, Brun Paola

机构信息

Department of Industrial Engineering, University of Padova, Padova, Italy.

Institute of Polymers, Composites and Biomaterials-National Research Council of Italy, Naples, Italy.

出版信息

PLoS One. 2015 Sep 11;10(9):e0137505. doi: 10.1371/journal.pone.0137505. eCollection 2015.

Abstract

The design of hybrid poly-ε-caprolactone (PCL)-self-assembling peptides (SAPs) matrices represents a simple method for the surface functionalization of synthetic scaffolds, which is essential for cell compatibility. This study investigates the influence of increasing concentrations (2.5%, 5%, 10% and 15% w/w SAP compared to PCL) of three different SAPs on the physico-chemical/mechanical and biological properties of PCL fibers. We demonstrated that physico-chemical surface characteristics were slightly improved at increasing SAP concentrations: the fiber diameter increased; surface wettability increased with the first SAP addition (2.5%) and slightly less for the following ones; SAP-surface density increased but no change in the conformation was registered. These results could allow engineering matrices with structural characteristics and desired wettability according to the needs and the cell system used. The biological and mechanical characteristics of these scaffolds showed a particular trend at increasing SAP concentrations suggesting a prevailing correlation between cell behavior and mechanical features of the matrices. As compared with bare PCL, SAP enrichment increased the number of metabolic active h-osteoblast cells, fostered the expression of specific osteoblast-related mRNA transcripts, and guided calcium deposition, revealing the potential application of PCL-SAP scaffolds for the maintenance of osteoblast phenotype.

摘要

混合聚己内酯(PCL)-自组装肽(SAPs)基质的设计是一种对合成支架进行表面功能化的简单方法,这对于细胞相容性至关重要。本研究调查了三种不同的SAPs浓度增加(相对于PCL,分别为2.5%、5%、10%和15% w/w的SAP)对PCL纤维的物理化学/机械和生物学特性的影响。我们证明,随着SAP浓度增加,物理化学表面特性略有改善:纤维直径增大;添加第一种SAP(2.5%)时表面润湿性增加,后续添加时增加幅度稍小;SAP表面密度增加,但构象未发生变化。这些结果可以根据需求和所使用的细胞系统设计具有特定结构特征和所需润湿性的基质。随着SAP浓度增加,这些支架的生物学和机械特性呈现出特定趋势,表明细胞行为与基质的机械特性之间存在显著相关性。与单纯的PCL相比,富含SAP增加了代谢活跃的人成骨细胞数量,促进了特定成骨细胞相关mRNA转录本的表达,并引导了钙沉积,揭示了PCL-SAP支架在维持成骨细胞表型方面的潜在应用。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c575/4567138/2268733f1b63/pone.0137505.g001.jpg

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