鉴定和表征人肾细胞中的 RNF2 反应元件。
Identification and characterization of RNF2 response elements in human kidney cells.
机构信息
Graduate School of Life Sciences and Biotechnology, Korea University, Seoul, 136-701, Korea.
出版信息
Mol Cells. 2011 Mar;31(3):247-53. doi: 10.1007/s10059-011-0033-7. Epub 2011 Jan 21.
Abstract
RING finger protein 2 (RNF2) contains a conserved N-terminal RING finger domain and functions as an E3 ligase. As a member of the Polycomb group family of proteins, RNF2 also represses a number of genes involved in development, differentiation, malignant transformation and cell cycle. Herein, using chromatin immunoprecipitation cloning, 33 RNF2-responding loci were identified in the genome of HEK293 human kidney cells. Luciferase reporter assays showed that among them, 26 and 2 loci acted as a repressor and an activator, respectively. RNA interference revealed that the identified RNF2-responding sequences regulated the transcriptional activity of nearby promoters. This study may contribute to elucidating the mechanism underlying RNF2-mediated transcriptional regulation.
摘要
环指蛋白 2（RNF2）含有一个保守的 N 端环指结构域，作为 E3 连接酶发挥作用。作为多梳蛋白家族的成员之一，RNF2 还抑制了许多参与发育、分化、恶性转化和细胞周期的基因。在此，通过染色质免疫沉淀克隆，在 HEK293 人肾细胞的基因组中鉴定出 33 个 RNF2 反应位点。荧光素酶报告基因检测显示，其中 26 个和 2 个位点分别作为抑制子和激活子发挥作用。RNA 干扰表明，所鉴定的 RNF2 反应序列调节了附近启动子的转录活性。本研究可能有助于阐明 RNF2 介导的转录调控的机制。
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