乙二醇诱导肾结石大鼠中长链非编码 RNA、mRNA 和环状 RNA 的表达谱变化。

Changing expression profiles of long non-coding RNAs, mRNAs and circular RNAs in ethylene glycol-induced kidney calculi rats.

机构信息

Department of Anesthesiology, Xiangya Hospital, Central South University, Xiangya Road 87#, Changsha, Hunan, 410008, People's Republic of China.

Department of Urology, Second Xiangya Hospital, Central South University, Changsha, Hunan, 410011, People's Republic of China.

出版信息

BMC Genomics. 2018 Sep 10;19(1):660. doi: 10.1186/s12864-018-5052-8.

BACKGROUND

To explore long non-coding RNA (lncRNA), mRNA and circular RNA (circRNA) expression profiles and their biological functions in the pathogenesis of kidney stones in ethylene glycol-induced urolithiasis rats.

RESULTS

The expression of 1440 lncRNAs, 2455 mRNAs and 145 circRNAs was altered in the kidneys of urolithiasis rats. GO and KEGG biological pathway analysis were performed to predict the functions of differentially expressed lncRNAs, circRNAs and co-expressed potential targeting genes. Co-expression networks of lncRNA-mRNA and circRNA-miRNA were constructed based on correlation analysis between differentially expressed RNAs. mRNAs coexpressed with lncRNAs were involved in many kidney diseases, e.g., Ephb6 was associated with the reabsorption ability of the kidney. Arl5b was associated with the dynamic changes in the podocyte foot process in podocyte injury. miRNAs co-expressed with circRNAs, such as rno-miR-138-5p and rno-miR-672-5p, have been proven to be functional in hypercalciuria urolithiasis.

CONCLUSION

The expression profile provided a systematic perspective on the potential functions of lncRNAs and circRNAs in the pathogenesis of kidney stones. Differentially expressed lncRNAs and circRNAs might serve as treatment targets for kidney stones.

背景

探讨乙二醇诱导的尿石症大鼠结石形成过程中长链非编码 RNA（lncRNA）、mRNA 和环状 RNA（circRNA）表达谱及其生物学功能。

结果

尿石症大鼠肾脏中 1440 个 lncRNA、2455 个 mRNA 和 145 个 circRNA 的表达发生了改变。GO 和 KEGG 生物通路分析用于预测差异表达的 lncRNA、circRNA 和共表达潜在靶向基因的功能。基于差异表达 RNA 之间的相关性分析，构建了 lncRNA-mRNA 和 circRNA-miRNA 的共表达网络。与 lncRNA 共表达的 mRNAs 参与许多肾脏疾病，例如 Ephb6 与肾脏的重吸收能力有关。Arl5b 与足细胞损伤中足突的动态变化有关。与 circRNAs 共表达的 miRNAs，如 rno-miR-138-5p 和 rno-miR-672-5p，已被证明在高钙尿结石形成中具有功能。