Department of Applied Biology and Chemical Technology, Institute of Modern Chinese Medicine, The Hong Kong Polytechnic University, Hong Kong, China.
J Ethnopharmacol. 2011 Apr 26;135(1):34-42. doi: 10.1016/j.jep.2011.02.017. Epub 2011 Feb 22.
The present study investigates the protective effects of water extract of ginseng (Panax ginseng C.A. Meyer) against 1-methyl-4-phenylpyridinium ion (MPP(+))-induced cytotoxicity in SH-SY5Y human neuroblastoma cells and explores the underlying mechanisms. The approach may be used for screening therapeutic agents for degenerative disorders such as Parkinson's disease.
SH-SY5Y human neuroblastoma cells were used to analyze the protective effects of water extract of ginseng (WEG) against multiple parameters such as MPP(+)-induced viability, oxidative injury, expression of Bax, Bcl-2, cytochrome c and cleaved caspase-3.
WEG exerted inhibitory effect on cell death, overproduction of ROS, elevated Bax/Bcl-2 ratio, release of cytochrome c and activation of caspase-3 expression in MPP(+)-treated SH-SY5Y cells.
WEG exhibited significant protective effects against MPP(+)-induced cytotoxicity in SH-SY5Y cells possibly through the suppression of ROS generation and the inhibition of mitochondria-dependent apoptotic pathway.
本研究旨在探讨人参水提物（Panax ginseng C.A. Meyer）对 1-甲基-4-苯基吡啶离子（MPP(+)）诱导的 SH-SY5Y 人神经母细胞瘤细胞毒性的保护作用，并探讨其潜在机制。该方法可用于筛选退行性疾病（如帕金森病）的治疗药物。
采用 SH-SY5Y 人神经母细胞瘤细胞分析人参水提物（WEG）对 MPP(+)诱导的细胞活力、氧化损伤、Bax、Bcl-2、细胞色素 c 和 cleaved caspase-3 表达等多种参数的保护作用。
WEG 对 MPP(+)处理的 SH-SY5Y 细胞的细胞死亡、ROS 过度产生、Bax/Bcl-2 比值升高、细胞色素 c 释放和 caspase-3 表达激活均有抑制作用。
WEG 对 MPP(+)诱导的 SH-SY5Y 细胞毒性具有显著的保护作用，可能通过抑制 ROS 的产生和抑制线粒体依赖的凋亡途径。
