National Center for Veterinary Drug Safety Evaluation, College of Veterinary Medicine, China Agricultural University, Beijing 100193, PR China.
Int J Food Microbiol. 2011 Mar 15;146(1):94-8. doi: 10.1016/j.ijfoodmicro.2011.01.035. Epub 2011 Feb 2.
The aim of this study was to determine the prevalence, antimicrobial resistance and molecular epidemiology of Campylobacter coli isolated from swine in China. A total of 190 C. coli isolates obtained from two slaughter houses and ten conventional pig farms in Shandong (SD, n=95) and Ningxia (NX, n=95) provinces were tested for their susceptibility to 14 antimicrobials. A high prevalence (>95%) of ciprofloxacin and tetracycline-resistant strains was observed in both SD and NX. The erythromycin and clindamycin resistance rates of C. coli from NX (ERY: 54.7% CLI: 43.2%) were higher than those from SD (ERY: 37.9%, CLI: 35.8%). A significant difference (P<0.05) was observed in erythromycin resistance rate, but not (P>0.05) in clindamycin resistance rate. while the resistance rates of ampicillin and kanamycin in NX (AMP: 34.7%, KAN: 43.2%) were significantly lower (P<0.05) than those in SD (AMP: 51.6%, KAN: 71.6%). None of the tested isolates were resistant to phenicols. The majority of the isolates from both provinces (SD: 80% and NX: 73.7%) showed multi-drug resistance profiles. The point mutations of A2075G in the 23S rRNA and C257T in the gyrA gene were detected in 98% (87/89) of macrolide resistant isolates and all ciprofloxacin resistant isolates, respectively. In addition, all tetracycline-resistant isolates harbored the tet(O) gene. The high prevalence of antimicrobial resistance in C. coli strains derived from pigs in China was observed and was likely due to the extensive use of various antimicrobials. Prudent use of antimicrobial agents on farms should be further emphasized to control the dissemination of antimicrobial resistant C. coli.
本研究旨在确定中国猪源结肠弯曲杆菌的流行率、抗菌药物耐药性和分子流行病学特征。共检测了来自山东（SD，n=95）和宁夏（NX，n=95）两省两个屠宰场和十个常规猪场的 190 株 C. coli 对 14 种抗菌药物的敏感性。SD 和 NX 中均观察到环丙沙星和四环素耐药菌株的高流行率（>95%）。NX 来源的 C. coli 对红霉素和克林霉素的耐药率（ERY：54.7% CLI：43.2%）高于 SD（ERY：37.9%，CLI：35.8%）。红霉素耐药率存在显著差异（P<0.05），但克林霉素耐药率无显著差异（P>0.05）。NX 中氨苄西林和卡那霉素的耐药率（AMP：34.7%，KAN：43.2%）显著低于 SD（AMP：51.6%，KAN：71.6%）。未检测到对苯氧青霉素耐药的菌株。两省分离株（SD：80%，NX：73.7%）均表现出多药耐药谱。23S rRNA 基因 A2075G 点突变和 gyrA 基因 C257T 点突变分别在 89%（87/98）大环内酯类耐药株和所有环丙沙星耐药株中检出。此外，所有四环素耐药株均携带 tet(O)基因。中国猪源结肠弯曲杆菌对各种抗菌药物的耐药率较高，这可能与抗菌药物的广泛应用有关。应进一步强调在农场谨慎使用抗菌药物，以控制耐药性 C. coli 的传播。
