Rawat Neelam, Kumar Deepak, Upadhyay A K
Department of Veterinary Public Health and Epidemiology, College of Veterinary and Animal Sciences, Govind Ballabh Pant University of Agriculture and Technology, Pantnagar, Uttarakhand, India.
Vet World. 2018 Dec;11(12):1698-1705. doi: 10.14202/vetworld.2018.1698-1705. Epub 2018 Dec 19.
Campylobacteriosis finds its place among the four important global foodborne illnesses. The disease, though self-limiting, needs antibacterial therapy in extraintestinal complications. Therefore, the present study was designed to estimate the prevalence of thermophilic in poultry, animals, and humans of the Kumaon region of Uttarakhand.
A total of 609 samples comprising of poultry ceca (n=116), poultry droppings (n=203), and feces of pigs (n=71), cattle (n=61), sheep (n=19), goat (n=17), human beings (n=88), and laboratory animals (n=34) (rats, rabbits, and guinea pigs) were collected. The thermophilic , and were confirmed using multiplex polymerase chain reaction. The isolates were also screened for the presence of virulence genes, and their antibiotic susceptibility testing was done against eight antibiotics.
An overall prevalence of 6.24% was revealed with highest from poultry ceca (15.52%), followed by poultry droppings (5.91%), cattle feces (4.92%), human stools (3.40%), and pig feces (2.82%). The virulence genes, namely , , , and , were present in 38 (100%), 37 (97.37%), 7 (18.42%), and 14 (36.84%) isolates, respectively. All the isolates were resistant to nalidixic acid, while all were sensitive to erythromycin and co-trimoxazole.
It was concluded that the animals and humans in the region harbored the thermophilic which may contribute to the human illness. Resistance shown among the isolates may complicate the antimicrobial therapy.
弯曲杆菌病位列全球四大重要食源性疾病之中。该疾病虽具有自限性，但在出现肠外并发症时需要抗菌治疗。因此，本研究旨在评估北阿坎德邦库马盎地区家禽、动物及人类中嗜热弯曲杆菌的流行情况。
共采集609份样本，包括家禽盲肠（n = 116）、家禽粪便（n = 203）、猪粪便（n = 71）、牛粪便（n = 61）、羊粪便（n = 19）、山羊粪便（n = 17）、人类粪便（n = 88）以及实验动物粪便（n = 34）（大鼠、兔子和豚鼠）。采用多重聚合酶链反应确认嗜热弯曲杆菌、空肠弯曲杆菌和结肠弯曲杆菌。对分离株还进行了毒力基因检测，并针对8种抗生素进行了药敏试验。
总体流行率为6.24%，其中家禽盲肠中最高（15.52%），其次是家禽粪便（5.91%）、牛粪（4.92%）、人类粪便（3.40%）和猪粪便（2.82%）。毒力基因，即ciaA、cadF、flaA和cdtA，分别存在于38株（100%）、37株（97.37%）、7株（18.42%）和14株（36.84%）分离株中。所有分离株对萘啶酸耐药，而对红霉素和复方新诺明均敏感。
得出结论，该地区的动物和人类携带嗜热弯曲杆菌，这可能导致人类患病。分离株表现出的耐药性可能会使抗菌治疗复杂化。
