Onagawa M, Ishihara K, Okuda K
Department of Microbiology, Tokyo Dental College, Chiba, Japan.
Bull Tokyo Dent Coll. 1994 Nov;35(4):171-81.
To elucidate an ecological profile of several periodontopathogens, the authors examined the coaggregation between cells of Porphyromonas gingivalis and oral bacterial strains including Treponema denticola in vitro. Coaggregation between cells of plaque bacteria was examined by visual assay and phase-contrast microscope. P. gingivalis cells coaggregated with strains of T. denticola and Treponema socranskii subspecies socranskii, but did not coaggregate with T. socranskii subspecies buccale, T. socranskii subspecies paredis, Treponema vincentii, or Treponema pectinovorum. The extracted hemagglutinin from P. gingivalis was active agglutinating T. denticola cells. Addition of serum and saliva somewhat affected the coaggregation, but no effects of tested sugars or amino acids were found. Heat treatment of T. denticola cells did not reduce the coagregation: heat treatment of P. gingivalis cells eliminated it. Growth inhibitory activity among these bacterial species was examined by the stab culture method. Strains of T. denticola ATCC 35404 and 35405 and T. vincentii inhibited the growth of some P. gingivalis strains, but not others. No strain of Treponema was inhibited by black-pigmented anaerobic rods. The coaggregation observed between P. gingivalis and T. denticola indicates the potential importance of their simultaneous existence in human periodontal pockets and development of the disease.
为了阐明几种牙周病原体的生态特征，作者在体外检测了牙龈卟啉单胞菌与包括齿垢密螺旋体在内的口腔细菌菌株细胞之间的共聚作用。通过视觉检测和相差显微镜检查菌斑细菌细胞之间的共聚作用。牙龈卟啉单胞菌细胞与齿垢密螺旋体菌株及索氏密螺旋体索氏亚种菌株发生共聚，但不与索氏密螺旋体颊亚种、索氏密螺旋体帕氏亚种、文森特密螺旋体或果胶分解密螺旋体发生共聚。从牙龈卟啉单胞菌中提取的血凝素能使齿垢密螺旋体细胞发生凝集。血清和唾液的添加对共聚作用有一定影响，但未发现受试糖类或氨基酸有影响。齿垢密螺旋体细胞的热处理并未降低凝集作用：牙龈卟啉单胞菌细胞的热处理则消除了凝集作用。通过穿刺培养法检测了这些细菌种类之间的生长抑制活性。齿垢密螺旋体ATCC 35404和35405菌株以及文森特密螺旋体抑制了一些牙龈卟啉单胞菌菌株的生长，但对其他菌株无抑制作用。没有一株密螺旋体受到黑色色素厌氧杆菌的抑制。牙龈卟啉单胞菌与齿垢密螺旋体之间观察到的共聚作用表明它们在人类牙周袋中同时存在以及疾病发展中具有潜在的重要性。
