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从牛子宫内膜细胞培养物中分离病毒。

Isolation of viruses from cultures of bovine endometrial cells.

作者信息

Luther P D, Nuttall P A, Gibbons R A

出版信息

J Infect Dis. 1978 Nov;138(5):660-3. doi: 10.1093/infdis/138.5.660.

DOI:10.1093/infdis/138.5.660
PMID:213504
Abstract

Endometrial cells from the uteri of pregnant and nonpregnant cattle were cultured. The presence of one or both of two viruses, noncytopathic mucosal disease virus and bovine syncytial virus, was demonstrated in seven of 19 endometria investigated. It was necessary to subculture the cells an average of four times to detect the viral infections. Difficulties were encountered in producing endometrial cell cultures from cows at term or near the end of term and also from older animals. The infections detected may be significant because both of the viruses isolated are capable of infecting the bovine fetus in utero and mucosal disease virus has been associated with bovine fetal diseases.

摘要

对怀孕和未怀孕母牛子宫的子宫内膜细胞进行了培养。在所研究的19个子宫内膜中,有7个检测出两种病毒中的一种或两种,即非细胞病变性黏膜病病毒和牛合胞体病毒。平均需要传代培养细胞4次才能检测到病毒感染。从足月或接近足月的母牛以及老年母牛获取子宫内膜细胞培养物时遇到了困难。检测到的感染可能具有重要意义,因为分离出的两种病毒都能够在子宫内感染牛胎儿,且黏膜病病毒与牛胎儿疾病有关。

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Isolation of viruses from cultures of bovine endometrial cells.从牛子宫内膜细胞培养物中分离病毒。
J Infect Dis. 1978 Nov;138(5):660-3. doi: 10.1093/infdis/138.5.660.
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Development of a cell culture line of bovine fetal endometrial cells.牛胎儿子宫内膜细胞系的建立。
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[Isolation of the bovine diarrhea virus].[牛腹泻病毒的分离]
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Detection by cultural isolation of bovine virus diarrhoea (BVD) virus following field infections in cattle and their fetuses in Turkey.通过培养分离法检测土耳其牛及其胎儿发生野外感染后牛病毒性腹泻(BVD)病毒的情况。
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[Detection of PI-3 viruses, BVD-MD viruses and parvoviruses in bovine fetuses after experimental intrauterine infections].[实验性宫内感染后牛胎儿中PI-3病毒、牛病毒性腹泻黏膜病病毒和细小病毒的检测]
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Viral contamination of bovine foetal serum and cell cultures.牛胎儿血清和细胞培养物的病毒污染
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