Unique gene-silencing and structural properties of 2'-fluoro-modified siRNAs.
作者信息
Manoharan Muthiah, Akinc Akin, Pandey Rajendra K, Qin June, Hadwiger Philipp, John Matthias, Mills Kathy, Charisse Klaus, Maier Martin A, Nechev Lubomir, Greene Emily M, Pallan Pradeep S, Rozners Eriks, Rajeev Kallanthottathil G, Egli Martin
机构信息
Alnylam Pharmaceuticals, 300 Third Street, Cambridge, MA 02142, USA.
出版信息
Angew Chem Int Ed Engl. 2011 Mar 1;50(10):2284-8. doi: 10.1002/anie.201006519. Epub 2011 Jan 31.
Abstract
摘要
相似文献
1
Unique gene-silencing and structural properties of 2'-fluoro-modified siRNAs.2'-氟修饰的小干扰RNA的独特基因沉默和结构特性。
Angew Chem Int Ed Engl. 2011 Mar 1;50(10):2284-8. doi: 10.1002/anie.201006519. Epub 2011 Jan 31.
2
Modified siRNAs for the study of the PAZ domain.经修饰的 siRNAs 用于 PAZ 结构域研究。
Chem Commun (Camb). 2010 Jun 28;46(24):4270-2. doi: 10.1039/c003221b. Epub 2010 May 19.
3
Contributions of 3'-overhang to the dissociation of small interfering RNAs from the PAZ domain: molecular dynamics simulation study.3'端突出对小干扰RNA从PAZ结构域解离的贡献：分子动力学模拟研究
J Mol Graph Model. 2007 Mar;25(6):784-93. doi: 10.1016/j.jmgm.2006.07.002. Epub 2006 Jul 11.
4
5΄-Vinylphosphonate improves tissue accumulation and efficacy of conjugated siRNAs in vivo.5΄-乙烯基膦酸酯可改善体内共轭小干扰RNA的组织蓄积及疗效。
Nucleic Acids Res. 2017 Jul 27;45(13):7581-7592. doi: 10.1093/nar/gkx507.
5
New opportunities for designing effective small interfering RNAs.设计有效小干扰 RNA 的新机会。
Sci Rep. 2019 Nov 6;9(1):16146. doi: 10.1038/s41598-019-52303-5.
6
Unexpected origins of the enhanced pairing affinity of 2'-fluoro-modified RNA.2'-氟修饰 RNA 增强配对亲和力的意外起源。
Nucleic Acids Res. 2011 Apr;39(8):3482-95. doi: 10.1093/nar/gkq1270. Epub 2010 Dec 22.
7
Crystal structure, stability and in vitro RNAi activity of oligoribonucleotides containing the ribo-difluorotoluyl nucleotide: insights into substrate requirements by the human RISC Ago2 enzyme.含核糖二氟甲苯基核苷酸的寡核糖核苷酸的晶体结构、稳定性及体外RNA干扰活性：对人RNA诱导沉默复合体AGO2酶底物需求的深入了解
Nucleic Acids Res. 2007;35(19):6424-38. doi: 10.1093/nar/gkm664. Epub 2007 Sep 18.
8
Structural insights into RISC assembly facilitated by dsRNA-binding domains of human RNA helicase A (DHX9).结构洞察 RISC 组装由人类 RNA 解旋酶 A（DHX9）的 dsRNA 结合域促进。
Nucleic Acids Res. 2013 Mar 1;41(5):3457-70. doi: 10.1093/nar/gkt042. Epub 2013 Jan 29.
9
Chemical modification of siRNA.小干扰RNA的化学修饰
Curr Opin Mol Ther. 2010 Apr;12(2):158-67.
10
Expanding the Scope of 2'-SCF3 Modified RNA.扩大2'-SCF3修饰RNA的范围。
Chemistry. 2015 Jul 13;21(29):10400-7. doi: 10.1002/chem.201500415. Epub 2015 Jun 12.
引用本文的文献
1
The Effect of 2'F-RNA on I-Motif Structure and Stability.2'F-RNA 对 I-基序结构和稳定性的影响。
Molecules. 2025 Aug 30;30(17):3561. doi: 10.3390/molecules30173561.
2
Chemical Modifications in Nucleic Acid Therapeutics.核酸疗法中的化学修饰
Methods Mol Biol. 2025;2965:57-126. doi: 10.1007/978-1-0716-4742-4_3.
3
The XNA alphabet.XNA字母表。
Nucleic Acids Res. 2025 Jul 8;53(13). doi: 10.1093/nar/gkaf635.
4
Systematic analysis of siRNA and mRNA features impacting fully chemically modified siRNA efficacy.影响完全化学修饰的小干扰RNA（siRNA）功效的siRNA和信使核糖核酸（mRNA）特征的系统分析
Nucleic Acids Res. 2025 Jun 20;53(12). doi: 10.1093/nar/gkaf479.
5
A first-in-class EGFR-directed KRAS G12V selective inhibitor.一种一流的表皮生长因子受体导向的KRAS G12V选择性抑制剂。
Cancer Cell. 2025 Jun 16. doi: 10.1016/j.ccell.2025.05.016.
6
Improving the potency prediction for chemically modified siRNAs through insights from molecular modeling of individual sequence positions.通过对单个序列位置的分子建模深入了解，提高化学修饰小干扰RNA的效力预测。
Mol Ther Nucleic Acids. 2024 Dec 5;36(1):102415. doi: 10.1016/j.omtn.2024.102415. eCollection 2025 Mar 11.
7
Technologies for Targeted RNA Degradation and Induced RNA Decay.靶向RNA降解与诱导RNA衰变技术
Chem Rev. 2024 Dec 11;124(23):13301-13330. doi: 10.1021/acs.chemrev.4c00472. Epub 2024 Nov 5.
8
Atom-Modified gDNA Enhances Cleavage Activity of TtAgo Enabling Ultra-Sensitive Nucleic Acid Testing.原子修饰 gDNA 增强了 TtAgo 的切割活性，使核酸检测达到超高灵敏度。
Adv Sci (Weinh). 2024 Jul;11(28):e2403120. doi: 10.1002/advs.202403120. Epub 2024 May 10.
9
Cholesterol-Modified Anti-Il6 siRNA Reduces the Severity of Acute Lung Injury in Mice.胆固醇修饰的抗 Il6 siRNA 可降低小鼠急性肺损伤的严重程度。
Cells. 2024 Apr 30;13(9):767. doi: 10.3390/cells13090767.
10
RNAi-based drug design: considerations and future directions.基于 RNAi 的药物设计：考虑因素和未来方向。
Nat Rev Drug Discov. 2024 May;23(5):341-364. doi: 10.1038/s41573-024-00912-9. Epub 2024 Apr 3.
本文引用的文献
1
Recent advances in small interfering RNA sensing by the immune system.免疫系统中对小干扰 RNA 的感应的最新进展。
N Biotechnol. 2010 Jul 31;27(3):236-42. doi: 10.1016/j.nbt.2010.02.015. Epub 2010 Feb 26.
2
Chemical modification of siRNA.小干扰RNA的化学修饰
Curr Protoc Nucleic Acid Chem. 2009 Dec;Chapter 16:Unit 16.3. doi: 10.1002/0471142700.nc1603s39.
3
The RNA-induced silencing complex: a versatile gene-silencing machine.RNA诱导沉默复合体：一种多功能的基因沉默机器。
J Biol Chem. 2009 Jul 3;284(27):17897-901. doi: 10.1074/jbc.R900012200. Epub 2009 Apr 1.
4
Development of lipidoid-siRNA formulations for systemic delivery to the liver.用于全身递送至肝脏的脂质体-siRNA制剂的研发。
Mol Ther. 2009 May;17(5):872-9. doi: 10.1038/mt.2009.36. Epub 2009 Mar 3.
5
Molecular imaging study on in vivo distribution and pharmacokinetics of modified small interfering RNAs (siRNAs).修饰后的小干扰RNA（siRNAs）体内分布及药代动力学的分子成像研究
Oligonucleotides. 2008 Sep;18(3):201-12. doi: 10.1089/oli.2008.0133.
6
The many roles for fluorine in medicinal chemistry.氟在药物化学中的多种作用。
J Med Chem. 2008 Aug 14;51(15):4359-69. doi: 10.1021/jm800219f. Epub 2008 Jun 21.
7
A combinatorial library of lipid-like materials for delivery of RNAi therapeutics.用于递送RNAi治疗药物的类脂质材料组合文库。
Nat Biotechnol. 2008 May;26(5):561-9. doi: 10.1038/nbt1402. Epub 2008 Apr 27.
8
Effective RNAi-mediated gene silencing without interruption of the endogenous microRNA pathway.有效的RNA干扰介导的基因沉默，且不干扰内源性微小RNA途径。
Nature. 2007 Oct 11;449(7163):745-7. doi: 10.1038/nature06179. Epub 2007 Sep 26.
9
RNA interference tolerates 2'-fluoro modifications at the Argonaute2 cleavage site.RNA干扰可耐受Argonaute2切割位点处的2'-氟修饰。
Chem Biodivers. 2007 May;4(5):858-73. doi: 10.1002/cbdv.200790073.
10
RNAi therapeutics: a potential new class of pharmaceutical drugs.RNA干扰疗法：一类潜在的新型药物。
Nat Chem Biol. 2006 Dec;2(12):711-9. doi: 10.1038/nchembio839.
Zotero 插件