Guo Liming, Zhang Shu, Zhao Jinhui, Zeng Xin, Hao Qiaoling, Luo Qifang, Wang Lin
Institute of Environment Medicine, School of Public Health, Tongji Medical College, Huazhong University of Science & Technology, MOE Key Lab of Environmental Health, Wuhan 430030, China.
Wei Sheng Yan Jiu. 2010 Nov;39(6):760-3.
To acclimate, screen the dominant bacteria for degrading pentachlorophenol and its growth characteristics and degradation conditions study.
Active sludge aeration and method of density gradient were used to acclimate the sludge. Using pentachlorophenol as a sole carbon source, it was increased step by step in continuous eight weeks. The biodegradation test on pentachlorophenol was conducted after isolation, purification of the dominant bacteria. The suitable temperature, the growth characteristics under different osmotic pressure and the proper degradation conditions of the dominant bacteria were investigated. According to the morphological characteristic, physiological and biochemical, the strain was preliminarily identified.
Three strains of dominant bacteria were screened. The degradation rates of one strain among them on pentachlorophenol could reach to 98.64% in 48 h. The degradation effect would be better when the temperature was 30 degrees C, pH was 6, and the concentration of pentachlorophenol was 0.2-0.4 g/L. The strain could grow best under 30 degrees C. It was halotolerant bacteria and identified as Acetobacter.
The dominant bacteria could be got through density gradient acclimatization in the active sludge aeration basin and its degradation effect on pentachlorophenol was obvious.
驯化、筛选出降解五氯苯酚的优势菌，并研究其生长特性及降解条件。
采用活性污泥曝气法和密度梯度法对污泥进行驯化。以五氯苯酚为唯一碳源，连续8周逐步递增。对优势菌进行分离、纯化后，进行五氯苯酚的生物降解试验。考察优势菌适宜的温度、不同渗透压下的生长特性及合适的降解条件。根据形态特征、生理生化特性对菌株进行初步鉴定。
筛选出三株优势菌。其中一株对五氯苯酚的降解率在48 h内可达98.64%。温度为30℃、pH为6、五氯苯酚浓度为0.2 - 0.4 g/L时，降解效果较好。该菌株在30℃下生长最佳。它是耐盐菌，鉴定为醋酸杆菌属。
通过活性污泥曝气池密度梯度驯化可获得优势菌，其对五氯苯酚的降解效果明显。
