MRC Centre for Medical Molecular Virology, Division of Infection and Immunity, University College London, 46 Cleveland St, London W1T 4JF, UK.
Retrovirology. 2011 Feb 25;8:13. doi: 10.1186/1742-4690-8-13.
XMRV is a gammaretrovirus associated in some studies with human prostate cancer and chronic fatigue syndrome. Central to the hypothesis of XMRV as a human pathogen is the description of integration sites in DNA from prostate tumour tissues. Here we demonstrate that 2 of 14 patient-derived sites are identical to sites cloned in the same laboratory from experimentally infected DU145 cells. Identical integration sites have never previously been described in any retrovirus infection. We propose that the patient-derived sites are the result of PCR contamination. This observation further undermines the notion that XMRV is a genuine human pathogen.
XMRV 是一种与某些研究中人类前列腺癌和慢性疲劳综合征相关的γ逆转录病毒。XMRV 作为人类病原体的假设的核心是描述来自前列腺肿瘤组织的 DNA 中的整合位点。在这里，我们证明，14 个患者来源的位点中有 2 个与在同一实验室中从实验性感染的 DU145 细胞中克隆的位点相同。在任何逆转录病毒感染中，从未描述过相同的整合位点。我们提出，患者来源的位点是 PCR 污染的结果。这一观察结果进一步削弱了 XMRV 是一种真正的人类病原体的观点。
