Babaei Farhad, Ahmadi Ali, Rezaei Farhad, Jalilvand Somayeh, Ghavami Nastaran, Mahmoudi Mahmoud, Abiri Ramin, Kondori Nasim, Nategh Rakhshande, Mokhtari Azad Talat
Department of Virology, School of Public Health, Tehran University of Medical Sciences, Tehran, IR Iran; Department of Microbiology, School of Medicine, Kermanshah University of Medical Sciences, Kermanshah, IR Iran.
Department of Pathology, School of Medicine, Tehran University of Medical Sciences, Tehran, IR Iran.
Iran Red Crescent Med J. 2015 Dec 19;17(12):e19439. doi: 10.5812/ircmj.19439. eCollection 2015 Dec.
Although several studies have confirmed the association of xenotropic murine leukemia virus-related virus (XMRV) and prostate cancer, this association is still controversial, as most studies did not detect XMRV in prostate tissue samples. Furthermore, some genetic and epidemiological studies have highlighted a role for RNase L polymorphisms, particularly R462Q, in the progression of prostate cancer.
The focus of this study was on the association of XMRV and RNase L R462Q variants with the risk of prostate cancer in Iranian patients.
In this case-control study, 40 and 80 individuals with sporadic prostate cancer and benign prostatic hyperplasia, respectively, were included. The presence of XMRV was evaluated by real-time polymerase chain reaction (PCR) of integrase and nested-PCR for the gag genes. The RNase L R462Q polymorphism analysis was carried out by PCR and sequencing.
In a total of 40 sporadic prostate cancer and 80 benign prostatic hyperplasia cases, no XMRV was detected by real-time PCR and nested-PCR. RNase L R462Q polymorphism analysis reveals that although there was an increase in the risk of prostate cancer correlated with the Q/Q allele of RNase L at position 462, the frequencies of the RNase L R462Q alleles were not statistically significant between the prostate cancer and benign prostatic hyperplasia groups (OR = 2.75 (95% CI = 0.67 - 11.3), P = 0.29).
These results did not support the presence of XMRV in the samples with prostate cancer and showed that RNase L R462Q variants had relatively little or no impact on the risk of prostate cancer in Iranian population.
尽管多项研究已证实嗜异性小鼠白血病病毒相关病毒（XMRV）与前列腺癌有关联，但这种关联仍存在争议，因为大多数研究在前列腺组织样本中未检测到XMRV。此外，一些遗传学和流行病学研究强调了核糖核酸酶L（RNase L）多态性，尤其是R462Q，在前列腺癌进展中的作用。
本研究的重点是探讨XMRV和RNase L R462Q变异与伊朗患者前列腺癌风险的关联。
在这项病例对照研究中，分别纳入了40例散发性前列腺癌患者和80例良性前列腺增生患者。通过整合酶的实时聚合酶链反应（PCR）和gag基因的巢式PCR评估XMRV的存在情况。通过PCR和测序进行RNase L R462Q多态性分析。
在总共40例散发性前列腺癌和80例良性前列腺增生病例中，实时PCR和巢式PCR均未检测到XMRV。RNase L R462Q多态性分析显示，尽管462位RNase L的Q/Q等位基因与前列腺癌风险增加相关，但前列腺癌组和良性前列腺增生组之间RNase L R462Q等位基因的频率无统计学意义（OR = 2.75（95%CI = 0.67 - 11.3），P = 0.29）。
这些结果不支持前列腺癌样本中存在XMRV，并表明RNase L R462Q变异对伊朗人群前列腺癌风险的影响相对较小或无影响。
