澳大利亚前列腺癌患者中 XMRV 或相关γ逆转录病毒与前列腺癌无关联。

No association between XMRV or related gammaretroviruses in Australian prostate cancer patients.

机构信息

Retroviral Biology and Antivirals Laboratory, Centre for Virology, Burnet Institute, 85 Commercial Road, Melbourne, Victoria 3004, Australia.

出版信息

Virol J. 2013 Jan 10;10:20. doi: 10.1186/1743-422X-10-20.

DOI:10.1186/1743-422X-10-20

PMID:23305518

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC3560155/

Abstract

BACKGROUND

Xenotropic murine leukemia virus-related virus (XMRV) is a gammaretrovirus reported to be associated with prostate cancer (PC) and chronic fatigue syndrome (CFS). While the association of XMRV with CFS and PC has recently been discredited, no studies have been performed in Australian patients to investigate the association between PC and XMRV or related murine leukemia virus (MLV) in matched PC and normal tissue.

METHODS

Genomic DNA (gDNA) was purified from matched normal and cancer formalin-fixed paraffin-embedded (FFPE) prostate tissue from 35 Australian PC patients with Gleason scores ranging from 7 - 10. The presence of the ribonuclease L (RNase L) polymorphism R462Q was determined by allele specific PCR. Samples were screened for XMRV and related murine leukemia virus (MLV) variants by qPCR. Contaminating mouse DNA was detected using qPCR targeting mouse intracisternal A particle long terminal repeat DNA.

RESULTS

gDNA was successfully purified from 94% (66/70) of normal and cancer FFPE prostate tissues. RNase L typing revealed 8% were homozygous (QQ), 60% were heterozygous (RQ) and 32% were wild-type (RR) for the RNase L mutation. None of the 66 samples tested were positive for XMRV or related MLV sequences using broad MLV or XMRV specific primers with detection sensitivities of 1 viral copy of MLV/XMRV and XMRV DNA, respectively.

CONCLUSIONS

Using highly sensitive qPCR we found no evidence of XMRV or related gammaretroviruses in prostate tissues from 35 Australian PC patients. Our findings are consistent with other studies demonstrating that XMRV is a laboratory contaminant that has no role in the aetiology of PC.

摘要

背景

嗜异性鼠白血病病毒相关病毒（XMRV）是一种γ逆转录病毒，据报道与前列腺癌（PC）和慢性疲劳综合征（CFS）有关。虽然 XMRV 与 CFS 和 PC 的关联最近已被否定，但尚未在澳大利亚患者中进行研究，以调查 PC 与 XMRV 或相关鼠白血病病毒（MLV）之间的关联，也没有在匹配的 PC 和正常组织中进行研究。

方法

从 35 名澳大利亚 PC 患者的匹配正常和癌症福尔马林固定石蜡包埋（FFPE）前列腺组织中提取基因组 DNA（gDNA），Gleason 评分范围为 7-10。通过等位基因特异性 PCR 确定核糖核酸酶 L（RNase L）多态性 R462Q 的存在。通过 qPCR 筛选 XMRV 和相关鼠白血病病毒（MLV）变体。使用针对小鼠内质网 A 颗粒长末端重复 DNA 的 qPCR 检测污染的小鼠 DNA。

结果

94%（66/70）的正常和癌症 FFPE 前列腺组织 gDNA 成功纯化。RNase L 分型显示 8%为纯合子（QQ），60%为杂合子（RQ），32%为野生型（RR）。使用 broad MLV 或 XMRV 特异性引物对 66 个样本进行检测，均未检测到 XMRV 或相关 MLV 序列，检测灵敏度分别为 MLV/XMRV 1 个病毒拷贝和 XMRV DNA。