Department of Microbiology and Immunology, Emory University School of Medicine, 1510 Clifton Rd, Atlanta, GA 30322, USA.
Virology. 2011 May 10;413(2):169-82. doi: 10.1016/j.virol.2011.01.027. Epub 2011 Feb 24.
We have utilized glycan microarray technology to determine the receptor binding properties of early isolates from the recent 2009 H1N1 human pandemic (pdmH1N1), and compared them to North American swine influenza isolates from the same year, as well as past seasonal H1N1 human isolates. We showed that the pdmH1N1 strains, as well as the swine influenza isolates examined, bound almost exclusively to glycans with α2,6-linked sialic acid with little binding detected for α2,3-linked species. This is highlighted by pair-wise comparisons between compounds with identical glycan backbones, differing only in the chemistry of their terminal linkages. The overall similarities in receptor binding profiles displayed by pdmH1N1 strains and swine isolates indicate that little or no adaptation appeared to be necessary in the binding component of HA for transmission from pig to human, and subsequent human to human spread.
我们利用糖链微阵列技术来确定最近的 2009 年 H1N1 人季节性流感大流行（pdmH1N1）早期分离株的受体结合特性，并将其与同年的北美猪流感分离株以及过去的季节性 H1N1 人分离株进行了比较。我们表明，pdmH1N1 株以及所检查的猪流感分离株几乎只与具有α2,6-连接唾液酸的糖链结合，而对α2,3-连接的糖链结合检测到的很少。这一点通过具有相同糖链骨架但末端连接化学性质不同的化合物之间的两两比较得到了突出显示。pdmH1N1 株和猪分离株显示的受体结合谱的总体相似性表明，在从猪到人传播以及随后的人际传播中，HA 的结合成分似乎不需要或很少需要适应。
