Department of Analytical Chemistry, Faculty of Chemistry, University of Seville, Prof. García González, s/n, 41012 Seville, Spain.
J Pharm Biomed Anal. 2011 May 15;55(2):332-41. doi: 10.1016/j.jpba.2011.01.037. Epub 2011 Feb 24.
In this paper, a three phase hollow fiber-based liquid phase microextraction (HF-LPME) combined with a HPLC procedure using diode array (DAD) and fluorescence detection (FLD) has been developed for the determination of eight widely used fluoroquinolones: marbofloxacin (MRB), norfloxacin (NRF), ciprofloxacin (CPR), danofloxacin (DNF), enrofloxacin (ENR), gatifloxacin (GTF), grepafloxacin (GRP) and flumequine (FLM). A Q3/2 Accurel PP polypropylene hollow fiber supporting 1-octanol was used between a 2 M Na2SO4 aqueous solution (pH 7) as donor phase and aqueous solution (pH 12) as acceptor phase. The microextraction parameters were optimised from an experimental central composite design. The procedure allows very low detection and quantitation limits of 0.3-16 ng L(-1) and 1-50 ng L(-1), respectively. The proposed method was applied to the determination of the analytes in bovine urine and in environmental water samples (surface, tap and wastewater).
本文采用高效液相色谱-二极管阵列检测法和荧光检测法，建立了一种基于三相中空纤维液相微萃取（HF-LPME）的分析方法，用于测定 8 种常用氟喹诺酮类药物：马波沙星（MRB）、诺氟沙星（NRF）、环丙沙星（CPR）、丹诺沙星（DNF）、恩诺沙星（ENR）、加替沙星（GTF）、格帕沙星（GRP）和氟甲喹（FLM）。实验中使用 Q3/2 Accurel PP 聚丙稀中空纤维作为支撑体，内充 1-辛醇，在 2 M 的硫酸钠水溶液（pH7）作为供相和 pH12 的水溶液作为受相之间形成三相体系。通过实验中心组合设计对微萃取参数进行优化。该方法的检测限和定量限分别为 0.3-16 ng L(-1)和 1-50 ng L(-1)。该方法应用于牛尿和环境水样（地表水、自来水和废水）中目标物的测定。
