College of Chemistry, Jilin University, Qianjin Street 2699, Changchun 130012, PR China.
Colloids Surf B Biointerfaces. 2011 Jun 1;84(2):484-90. doi: 10.1016/j.colsurfb.2011.02.003. Epub 2011 Feb 25.
In this paper, surface plasmon resonance biosensors based on magnetic core/shell Fe(3)O(4)/SiO(2) and Fe(3)O(4)/Ag/SiO(2) nanoparticles were developed for immunoassay. With Fe(3)O(4) and Fe(3)O(4)/Ag nanoparticles being used as seeding materials, Fe(3)O(4)/SiO(2) and Fe(3)O(4)/Ag/SiO(2) nanoparticles were formed by hydrolysis of tetraethyl orthosilicate. The aldehyde group functionalized magnetic nanoparticles provide organic functionality for bioconjugation. The products were characterized by scanning electronic microscopy (SEM), transmission electronic microscopy (TEM), FTIR and UV-vis absorption spectrometry. The magnetic nanoparticles possess the unique superparamagnetism property, exceptional optical properties and good compatibilities, and could be used as immobilization matrix for goat anti-rabbit IgG. The magnetic nanoparticles can be easily immobilized on the surface of SPR biosensor chip by a magnetic pillar. The effects of Fe(3)O(4)/SiO(2) and Fe(3)O(4)/Ag/SiO(2) nanoparticles on the sensitivity of SPR biosensors were also investigated. As a result, the SPR biosensors based on Fe(3)O(4)/SiO(2) nanoparticles and Fe(3)O(4)/Ag/SiO(2) nanoparticles exhibit a response for rabbit IgG in the concentration range of 1.25-20.00 μg ml(-1) and 0.30-20.00 μg ml(-1), respectively.
本文基于磁性核壳 Fe(3)O(4)/SiO(2) 和 Fe(3)O(4)/Ag/SiO(2) 纳米粒子，开发了用于免疫分析的表面等离子体共振生物传感器。以 Fe(3)O(4) 和 Fe(3)O(4)/Ag 纳米粒子为种子材料，通过正硅酸乙酯的水解形成 Fe(3)O(4)/SiO(2) 和 Fe(3)O(4)/Ag/SiO(2) 纳米粒子。醛基功能化磁性纳米粒子为生物偶联提供了有机功能。通过扫描电子显微镜 (SEM)、透射电子显微镜 (TEM)、傅里叶变换红外光谱 (FTIR) 和紫外可见吸收光谱对产物进行了表征。磁性纳米粒子具有独特的超顺磁性、优异的光学性质和良好的相容性，可用作山羊抗兔 IgG 的固定化基质。磁性纳米粒子可通过磁柱轻松固定在 SPR 生物传感器芯片的表面。还研究了 Fe(3)O(4)/SiO(2) 和 Fe(3)O(4)/Ag/SiO(2) 纳米粒子对 SPR 生物传感器灵敏度的影响。结果表明，基于 Fe(3)O(4)/SiO(2) 纳米粒子和 Fe(3)O(4)/Ag/SiO(2) 纳米粒子的 SPR 生物传感器分别在 1.25-20.00 μg ml(-1)和 0.30-20.00 μg ml(-1)范围内对兔 IgG 有响应。
