Philipps-Universität Marburg, Institut für Pharmazeutische Biologie, Deutschhausstr. 17A, D-35037 Marburg, Germany.
Phytochemistry. 2011 May;72(7):557-63. doi: 10.1016/j.phytochem.2011.01.036. Epub 2011 Feb 24.
Equisetum arvense L. (Equisetaceae-horsetail) accumulates various flavones and flavonols in infertile shoot. Enzyme assays conducted with crude extracts of the green tissue revealed chalcone synthase activity and also three further activities assigned to flavonoid biosynthesis and identified as flavone synthase I, flavanone 3β-hydroxylase and flavonol synthase. The latter three activities were characterized as soluble, 2-oxoglutarate-dependent dioxygenases by their typical cofactor requirements and peculiar inhibition. Notably, this is the first report of flavone synthase I which had been considered to be restricted solely to species of the Apiaceae from a distant plant taxon.
问荆（木贼科）在不育枝中积累了各种黄酮类和黄酮醇类物质。对绿色组织的粗提物进行的酶分析显示出查尔酮合酶的活性，以及另外三种被分配到类黄酮生物合成中的活性，被鉴定为黄酮醇合酶、黄酮醇合酶和 flavanone 3β-羟化酶。后三种活性被认为是可溶性的，2-氧戊二酸依赖性加双氧酶，其典型的辅助因子需求和特殊的抑制作用。值得注意的是，这是黄酮醇合酶 I 的首次报道，它被认为仅局限于远缘植物类群的伞形科植物。
