College of Chemistry, Chemical Engineering and Biotechnology, Donghua University, 2999 North Remin Road, Songjiang University City, Shanghai 201620, China.
Colloids Surf B Biointerfaces. 2011 Jun 1;84(2):477-83. doi: 10.1016/j.colsurfb.2011.02.002. Epub 2011 Feb 26.
Thermosensitive core-shell magnetic composite particles with a magnetic silica core and a rich poly (N-vinylcaprolactam) (PNVCL) shell layer were developed for studying the adsorption of bovine serum albumin (BSA) in a batch system. Various analytical and spectroscopic techniques including SEM, FT-IR, VSM and DSC were used to characterize the adsorbents prepared in this study. The combined effects of operating parameters such as initial temperature, pH and initial BSA concentration on the adsorption were analyzed using response surface methodology. The optimum conditions were 40°C, pH 4.68, and initial BSA concentration 2.0 mg/mL. Desorption experiments were conducted by altering the system temperature where a high recovery rate of protein was obtained. The separation process developed here indicates that the dual-responsive smart adsorbent could be an ideal candidate for the separation of protein.
研究了具有磁性二氧化硅核和丰富的聚（N-乙烯基己内酰胺）（PNVCL）壳层的温敏核壳磁性复合粒子，用于在批处理系统中研究牛血清白蛋白（BSA）的吸附。使用 SEM、FT-IR、VSM 和 DSC 等各种分析和光谱技术对本研究中制备的吸附剂进行了表征。使用响应面法分析了初始温度、pH 和初始 BSA 浓度等操作参数对吸附的综合影响。最佳条件为 40°C、pH 4.68 和初始 BSA 浓度 2.0mg/mL。通过改变系统温度进行解吸实验，得到了较高的蛋白质回收率。这里开发的分离过程表明，这种双响应智能吸附剂可能是蛋白质分离的理想候选物。
