Korea Institute of Ceramic Engineering and Technology, Seoul 153-801, South Korea.
Anal Biochem. 2010 Oct 1;405(1):135-7. doi: 10.1016/j.ab.2010.05.027. Epub 2010 Jun 1.
A novel approach to the hydrophobic partitioning effect on efficient separation of protein such as BSA was demonstrated by the modification of hydrophobic pockets on the surface of silica-coated magnetic nanoparticles with various alkyl groups at various pH levels. The separation efficiency is strongly reflected and can be attained by controlling the size of the hydrophobic pocket and other factors such as the alkyl chain length, the salt concentration, and the pH levels. Furthermore, the adsorption constant (k(ad)) was calculated for the hydrophobic partitioning interaction between BSA and the hydrophobic pocket size at various pH levels.
通过在二氧化硅包覆的磁性纳米粒子表面修饰具有不同烷基的疏水性口袋，在不同 pH 值下展示了一种对蛋白质（如 BSA）有效分离的疏水分配效应的新方法。分离效率强烈地反映出来，并可以通过控制疏水性口袋的大小和其他因素来实现，如烷基链长度、盐浓度和 pH 值。此外，还计算了在不同 pH 值下 BSA 与疏水性口袋大小之间的疏水分配相互作用的吸附常数（k(ad)）。
