dsRNA 在粗糙脉孢菌中的反应的一位记者。

A reporter for dsRNA response in Neurospora crassa.

机构信息

State Key Laboratory of Agrobiotechnology, College of Biological Sciences, China Agricultural University, Beijing 100193, China.

出版信息

FEBS Lett. 2011 Mar 23;585(6):906-12. doi: 10.1016/j.febslet.2011.02.024. Epub 2011 Feb 25.

DOI:10.1016/j.febslet.2011.02.024

PMID:21354420

Abstract

In the filamentous fungus Neurospora, the production of dsRNA can elicit a dsRNA-induced transcriptional response similar to the interferon response in vertebrates. However, how fungi sense the expression of dsRNA and activate gene expression is unknown. In this study, we established a dsRNA response reporter system in Neurospora crassa. Using the dsRNA-activated RNA-dependent RNA polymerase gene rrp-3 promoter, we created an expression construct (pRRP-3::Myc-Al-1) and introduced it into al-1(KO) mutant. The test dsRNA efficiently induced pRRP-3::Myc-Al-1 expression in the al-1(KO) mutant, resulting in conidia color switching from white to yellow. These results confirm that the dsRNA response is regulated at the transcriptional level and this reporter system can be used for future studies in dsRNA response in filamentous fungi.

摘要

在丝状真菌 Neurospora 中，dsRNA 的产生可以引发类似于脊椎动物干扰素反应的 dsRNA 诱导的转录反应。然而，真菌如何感知 dsRNA 的表达并激活基因表达尚不清楚。在本研究中，我们在 Neurospora crassa 中建立了一个 dsRNA 反应报告系统。使用 dsRNA 激活的 RNA 依赖性 RNA 聚合酶基因 rrp-3 启动子，我们创建了一个表达构建体（pRRP-3::Myc-Al-1）并将其引入 al-1(KO)突变体。测试 dsRNA 有效地诱导了 al-1(KO)突变体中 pRRP-3::Myc-Al-1 的表达，导致分生孢子颜色从白色变为黄色。这些结果证实 dsRNA 反应是在转录水平上调控的，并且这个报告系统可以用于丝状真菌 dsRNA 反应的未来研究。