粗糙脉孢菌基因缺失菌株的表型分析。

Phenotypic analysis of Neurospora crassa gene deletion strains.

作者信息

Turner Gloria E

机构信息

Department of Chemistry and Biochemistry, University of California, Los Angeles, CA, USA.

出版信息

Methods Mol Biol. 2011;722:191-8. doi: 10.1007/978-1-61779-040-9_14.

DOI:10.1007/978-1-61779-040-9_14

PMID:21590422

Abstract

Phenotypic analysis of Neurospora crassa knockout (KO) mutants was used as a vehicle to introduce students to laboratory research. The availability of gene deletion strains was the impetus for the development of a program designed to introduce beginning science students to basic microbiology, genetics, microscopy and beginning bioinformatics. The goal was to provide a research experience, acquire laboratory skills and phenotype hundreds of KO mutants. The data provided by the students was used to build a phenotype database at the Broad Institute at Harvard/MIT for the fungal scientific community. Each mutant analysis consists of five assays that examine growth and morphology, asexual and sexual development using wild-type (parental) strains as a reference. This information indicates how loss of each gene impacts these basic and important processes.

摘要

粗糙脉孢菌基因敲除（KO）突变体的表型分析被用作引导学生进行实验室研究的载体。基因缺失菌株的可得性推动了一个项目的开展，该项目旨在向刚开始学习科学的学生介绍基础微生物学、遗传学、显微镜技术和基础生物信息学。目标是提供研究经验、培养实验室技能并对数百个基因敲除突变体进行表型分析。学生提供的数据被用于在哈佛/麻省理工学院的布罗德研究所为真菌科学界建立一个表型数据库。每个突变体分析包括五项检测，这些检测以野生型（亲本）菌株作为参照，来研究生长与形态、无性和有性发育。这些信息表明每个基因的缺失如何影响这些基本且重要的过程。