Dipartimento di Biopatologia e Biotecnologie Mediche e Forensi, Università di Palermo, Corso Tukory 211, 90131 Palermo, Italy.
Res Vet Sci. 2011 Dec;91(3):e64-7. doi: 10.1016/j.rvsc.2011.01.029. Epub 2011 Feb 26.
A method to assess the expansion of antigen-specific intracellular IFN-γ positive T cell subsets during the infection will be helpful for a better understanding of mycoplasmal infections physiopathology in the sheep. We analysed the percentage of antigen-specific lymphocytes positive for intracellular IFN-γ during the infection of sheep with Mycoplasma agalactiae by culturing peripheral blood mononuclear cells of infected or uninfected animals with irradiated M. agalactiae. The expansion of antigen-specific IFN-γ positive lymphocytes in infected sheep was initially sustained by CD4(+) T cells at day 15 after infection, when antigen specific IgG start to be detectable, followed by CD8/IFN-γ double positive cells. γδ T-cells were not expanded at any time point analysed. IFNγ(+) T cells disappear 60 days after infection, suggesting that antigen specific IFNγ(+) T cells, mainly detected in the early phase of the disease, could be useful to understand the role of cell-mediated immunity during M. agalactiae infection.
一种评估感染期间抗原特异性细胞内 IFN-γ 阳性 T 细胞亚群扩增的方法将有助于更好地了解绵羊支原体感染的病理生理学。我们通过用照射过的 M. agalactiae 培养感染或未感染动物的外周血单核细胞，分析了绵羊感染 M. agalactiae 期间细胞内 IFN-γ 阳性淋巴细胞的百分比。在感染后 15 天，当可检测到抗原特异性 IgG 时，感染绵羊中抗原特异性 IFN-γ 阳性淋巴细胞的扩增最初由 CD4(+)T 细胞维持，随后是 CD8/IFN-γ 双阳性细胞。在分析的任何时间点，γδ T 细胞均未扩增。感染后 60 天 IFNγ(+)T 细胞消失，提示在疾病早期主要检测到的抗原特异性 IFNγ(+)T 细胞可能有助于了解细胞介导免疫在 M. agalactiae 感染中的作用。
