Volcán G S, Medrano C E
Departamento de Parasitología y Microbiología, Escuela de Medicina, Universidad de Oriente, Ciudad Bolívar, Venezuela.
Rev Inst Med Trop Sao Paulo. 1990 Nov-Dec;32(6):395-402. doi: 10.1590/s0036-46651990000600002.
Specimens of Dasyprocta leporina (Linnaeus, 1758) were raised out their natural environment. At three to four months of age they were orally inoculated with Lagochilascaris minor (Leiper, 1909) eggs obtained from a native patient. The eggs were incubated for more than 80 days so that it was possible to obtain, by mechanical compression, larvae that could be maintained alive in liquid medium for 48 hours or more. The animals were sacrificed 14-46 days after infection and tangled larva in inflammatory nodules were found in skeletal muscle without foreign body reaction, abscess formation or calcification. The development of the nodules did not seem to affect the hosts. The larvae obtained were similar to those described by SPRENT as the third stage of these helminths. When white mice were inoculated with similar material it was not possible to recuperate larva from their tissues nor were nodules found. Based on these results it is postulated that the helminth does not present a pulmonary cycle and that its development requires an intermediary host.
从自然环境中取出棉鼠（Dasyprocta leporina，林奈，1758年）的样本。在它们三到四个月大时，经口接种从一名当地患者身上获取的微小唇蛔线虫（Lagochilascaris minor，莱珀，1909年）卵。这些卵孵化超过80天，以便通过机械挤压获得能够在液体培养基中存活48小时或更长时间的幼虫。在感染后14 - 46天处死动物，在骨骼肌中发现缠结在炎性结节中的幼虫，无异物反应、脓肿形成或钙化。结节的形成似乎并未影响宿主。所获得的幼虫与斯普伦特描述的这些蠕虫第三阶段的幼虫相似。当用类似材料接种小白鼠时，无法从其组织中回收幼虫，也未发现结节。基于这些结果推测，该蠕虫不存在肺循环，其发育需要中间宿主。
