Luna-Olivares Luz Adilia, Kyvsgaard Niels Chr, Ferdushy Tania, Nejsum Peter, Thamsborg Stig Milan, Roepstorff Allan, Iburg Tine Moesgaard
Section for Parasitology and Aquatic Diseases, Deparment of Veterinary Disease Biology, Faculty of Health and Medical Sciences, University of Copenhagen, Thorvaldsensvej 57, 1871, Frederiksberg, Copenhagen, Denmark,
Parasitol Res. 2015 Jul;114(7):2507-15. doi: 10.1007/s00436-015-4450-y. Epub 2015 Apr 17.
This histopathological study was carried out in order to investigate the cellular response in the jejunum to Ascaridia galli during the first 7 weeks of infection. Fourty-two ISA Brown chickens (7 weeks old) were infected orally with 500 embryonated A. galli eggs each while 28 chickens were left as uninfected controls. Six infected and four control chickens were necropsied at each time point 3, 7, 10, 14, 21, 28 and 42 days post-infection (dpi). Samples for histopathology were taken from three sites of the jejunoileum. Significantly higher eosinophil counts were seen in infected chickens compared to uninfected at 3, 7, 10, 14 and 28 dpi (P < 0.01). In both groups, the initial number of mast cells was high, but this high level of mast cells remained for a longer period in the infected group compared to the control group. Significantly higher counts were thus found in the infected group at 21 (P < 0.001), 28 (P < 0.01) and 42 dpi (P < 0.05). A. galli infection induced changes in the mucosal thickness as reduced villi length at 7, 10, 14, 21 and 28 dpi and in the degree of general cellular infiltration in the lamina propria of the mucosal layer. No adult worms were seen during the experiment; therefore, A. galli larvae have elicited a moderate cellular response in the lamina propria, mainly consisting of eosinophils in the early phase and later of mast cells.
进行这项组织病理学研究是为了调查空肠在感染鸡蛔虫的前7周内的细胞反应。42只ISA褐壳蛋鸡（7周龄）每只经口感染500枚受精的鸡蛔虫卵，同时将28只鸡作为未感染对照。在感染后3、7、10、14、21、28和42天（dpi）的每个时间点对6只感染鸡和4只对照鸡进行剖检。从空肠回肠的三个部位采集组织病理学样本。在感染后3、7、10、14和28天，感染鸡的嗜酸性粒细胞计数显著高于未感染鸡（P＜0.01）。在两组中，肥大细胞的初始数量都很高，但与对照组相比，感染组肥大细胞的高水平持续时间更长。因此，在感染组中，在21天（P＜0.001）、28天（P＜0.01）和42天（P＜0.05）时发现计数显著更高。鸡蛔虫感染导致黏膜厚度发生变化，在感染后7、10、14、21和28天绒毛长度缩短，并且黏膜层固有层的一般细胞浸润程度也发生变化。在实验过程中未见到成虫；因此，鸡蛔虫幼虫在固有层引发了适度的细胞反应，早期主要由嗜酸性粒细胞组成，后期由肥大细胞组成。
