Zhang Jifen, Wang Qin, Jia Lan, Zhang Yu, Xu Xiaoyu
College of Pharmaceutical Sciences, Southwest University, Chongqing 400715, China.
Zhongguo Zhong Yao Za Zhi. 2010 Nov;35(22):2972-5. doi: 10.4268/cjcmm20102208.
To construct the ferulic acid loaded chitosan microspheres containing liposomes.
Ferulic acid was selected to be the model drug. Liposomes were prepared by calcium acetate gradient method. The entrapment efficiency of liposomes was(79.97 +/- 0.54)%, the average size was (187.6 +/- 11.9) nm and the Zeta potential was -12.67 +/-1.78. The chitosan microspheres, whose entrapment efficiency was (57.89 +/- 1.72)%, were prepared by onic gelation method after liposomes were mixed with chiotosan solution. Ferulic acid was entrapped in liposomes in amorphous form and then liposomes were distributed in microspheres intactly.
The 80.97% liposomes were released from microspheres and the 32.33% ferulic acid was released from microspheres at 12 h, which was much slower than simple chitosan microspheres.
This study demonstrated that chitosan microspheres containing liposomes have good sustained-releasing property in vitro.
构建载有阿魏酸的脂质体壳聚糖微球。
选用阿魏酸作为模型药物。采用醋酸钙梯度法制备脂质体。脂质体的包封率为（79.97±0.54）%，平均粒径为（187.6±11.9）nm，Zeta电位为-12.67±1.78。脂质体与壳聚糖溶液混合后，采用离子凝胶法制备壳聚糖微球，其包封率为（57.89±1.72）%。阿魏酸以无定形形式包封于脂质体中，然后脂质体完整地分布于微球中。
12 h时，80.97%的脂质体从微球中释放，32.33%的阿魏酸从微球中释放，这比单纯壳聚糖微球的释放速度慢得多。
本研究表明，载有脂质体的壳聚糖微球在体外具有良好的缓释性能。
