Carbone Claudia, Caddeo Carla, Grimaudo Maria Aurora, Manno Daniela Erminia, Serra Antonio, Musumeci Teresa
Laboratory of Drug Delivery Technology, Department of Drug Sciences, University of Catania, viale A. Doria 6, 95125 Catania, Italy.
Department of Scienze della Vita e dell'Ambiente, University of Cagliari, via Ospedale 72, 09124 Cagliari, Italy.
Nanomaterials (Basel). 2020 May 8;10(5):898. doi: 10.3390/nano10050898.
Nowadays, an increasing interest in combinatorial drug delivery systems is emerging, highlighting the possibility of exploiting essential oils (EO) for topical applications. This work aimed at developing nanostructured lipid carriers (NLC) for the combined delivery of ferulic acid and EO, whose beneficial effects in wound-healing processes have been widely reported. Homogeneous (polydispersity index, PDI < 0.2) nanoparticles with a small size (<150 nm) and a high encapsulation efficiency (>85%) were obtained. The co-presence of ferulic acid and EO, as compared to synthetic isopropyl myristate-based NLC, increased nanoparticles' stability, due to higher ordering chains, as confirmed by morphological and physicochemical studies. An enhanced cytocompatibility was observed when combining ferulic acid and EO, as confirmed by in vitro studies on fibroblasts. Furthermore, the combined delivery of ferulic acid and EO significantly promoted cell migration with higher effectiveness in respect to the free drug solution and the carrier without the EO. Taken all together, our results suggest a potential combined effect of the antioxidant ferulic acid and EO co-delivered in lipid nanoparticles in promoting cell proliferation and migration, representing a promising strategy in the treatment of wounds.
如今，人们对组合药物递送系统的兴趣日益浓厚，这凸显了利用精油（EO）进行局部应用的可能性。这项工作旨在开发用于联合递送阿魏酸和EO的纳米结构脂质载体（NLC），其在伤口愈合过程中的有益作用已被广泛报道。获得了尺寸小（<150 nm）、多分散指数（PDI）<0.2且包封效率高（>85%）的均匀纳米颗粒。形态学和物理化学研究证实，与基于肉豆蔻酸异丙酯的合成NLC相比，阿魏酸和EO的共存提高了纳米颗粒的稳定性，这是由于链的有序性更高。体外成纤维细胞研究证实，联合使用阿魏酸和EO时观察到细胞相容性增强。此外，与游离药物溶液和不含EO的载体相比，阿魏酸和EO的联合递送显著促进细胞迁移，效果更佳。综上所述，我们的结果表明，抗氧化剂阿魏酸和EO在脂质纳米颗粒中联合递送对促进细胞增殖和迁移具有潜在的联合作用，这代表了一种治疗伤口的有前景的策略。
