Brewer David M, Cerda-Gonzalez Sofia, Dewey Curtis W, Diep Anh N, Van Horne Kristin, McDonough Sean P
Department of Clinical Sciences, Cornell University, Ithaca, NY 14853, USA.
J Am Vet Med Assoc. 2011 Mar 1;238(5):618-24. doi: 10.2460/javma.238.5.618.
To evaluate clinical features and outcome of dogs with a confirmed spinal cord nephroblastoma and to describe the use of Wilms tumor-1 (WT-1) immunohistochemical staining to confirm a diagnosis of nephroblastoma in dogs.
Retrospective case series. Animals-11 dogs with a spinal cord nephroblastoma.
Medical records of dogs with a spinal cord nephroblastoma were reviewed. Information extracted included signalment, history, clinical signs, results of diagnostic testing, tumor location, treatment, and outcome. The diagnosis was confirmed through histologic review and WT-1 immunohistochemical staining of a tumor sample. In dogs with negative results for staining with WT-1, staining for cytokeratin, vimentin, and glial fibrillar acidic protein was performed.
11 dogs had a spinal cord tumor with a histologic appearance and immunohistochemical staining consistent with a nephroblastoma. Positive results for staining with WT-1 were detected in 9 of 11 dogs. Age at admission ranged from 5 to 48 months (median, 14 months). Nine dogs were female. All had progressive paraparesis, paraplegia, or ataxia. Duration of clinical signs ranged from 2 to 60 days (median, 14 days). Median survival time was 30 days from the time of diagnosis. Median survival time in dogs treated via surgical resection was 70.5 days.
The prognosis for dogs with a spinal cord nephroblastoma appeared to be poor, although combined surgical resection and radiation therapy may provide a good functional outcome. Results for staining with WT-1 can be used to support a diagnosis of nephroblastoma.
评估确诊为脊髓肾母细胞瘤犬的临床特征及预后，并描述应用威尔姆斯瘤-1（WT-1）免疫组化染色确诊犬肾母细胞瘤的情况。
回顾性病例系列研究。动物——11只患有脊髓肾母细胞瘤的犬。
查阅患有脊髓肾母细胞瘤犬的病历。提取的信息包括特征、病史、临床症状、诊断性检查结果、肿瘤位置、治疗及预后。通过对肿瘤样本进行组织学检查和WT-1免疫组化染色来确诊。对于WT-1染色结果为阴性的犬，进行细胞角蛋白、波形蛋白和胶质纤维酸性蛋白的染色。
11只犬患有脊髓肿瘤，其组织学表现和免疫组化染色结果与肾母细胞瘤一致。11只犬中有9只WT-1染色呈阳性。入院时年龄为5至48个月（中位数为14个月）。9只为雌性。所有犬均有进行性轻截瘫、截瘫或共济失调。临床症状持续时间为2至60天（中位数为14天）。从诊断时起，中位生存时间为30天。经手术切除治疗的犬中位生存时间为70.5天。
脊髓肾母细胞瘤犬的预后似乎较差，尽管手术切除联合放疗可能会带来良好的功能预后。WT-1染色结果可用于支持肾母细胞瘤的诊断。
