Department of Animal Production, University of Lleida, Lleida, Spain.
Theriogenology. 2011 Jun;75(9):1695-8. doi: 10.1016/j.theriogenology.2011.01.006. Epub 2011 Feb 26.
Intrafollicular insemination (IFI) is based on direct introduction of a sperm suspension into a preovulatory follicle. To our knowledge, the first cases of IFI resulting in pregnancy of the cow are reported here. The experiment was performed on a dairy herd with low fertility during the warm season of the year. Following the sequence of estrus detection, one in three inseminations was intrafollicular (n = 17), whereas deposition of semen was performed into the uterine body (IUI) in the remaining cows (n = 33). Approximately 0.06 ml of a seminal dose containing five million spermatozoa (one quarter of a commercial seminal dose in a 0.25 ml French straw) was injected trans-vaginally into the preovulatory follicle in the IFI group. Four (23.5%) and 3 (9%) cows of the IFI and IUI groups, respectively, became pregnant. On days 8-11 post-insemination, ovulation failure was registered in 3 (18%) and in 4 (12%) cows of the IFI and IUI groups, respectively. Our results suggest that IFI could be used as an alternative procedure to the usual deposition of semen into the uterus in cows of low fertility.
卵泡内授精（IFI）是基于将精子悬浮液直接引入到一个排卵前的卵泡中。据我们所知，这里首次报道了卵泡内授精导致奶牛怀孕的情况。该实验在一个奶牛群中进行，该奶牛群在一年中的温暖季节生育力较低。在检测到发情的顺序后，三分之一的授精是卵泡内授精（n=17），而其余的奶牛（n=33）则将精液沉积到子宫体中。在 IFI 组中，通过阴道将大约 0.06 毫升的精液剂量（商业精液剂量的四分之一，在 0.25 毫升的法国 straw 中）注入到排卵前的卵泡中。IFI 组和 IUI 组的 4 头（23.5%）和 3 头（9%）奶牛分别怀孕。在授精后 8-11 天，分别有 3 头（18%）和 4 头（12%）的 IFI 组和 IUI 组的奶牛发生了排卵失败。我们的结果表明，IFI 可以作为一种替代方法，用于生育力较低的奶牛中通常将精液沉积到子宫中的方法。
