Fleisch Valerie C, Jametti Tiziana, Neuhauss Stephan C F
Institute of Zoology, Neurobiology, University of Zurich, 8057 Zurich, Switzerland.
CSH Protoc. 2008 Mar 1;2008:pdb.prot4973. doi: 10.1101/pdb.prot4973.
INTRODUCTIONThe electroretinogram (ERG) is an electrophysiological tool used to measure electrical activity originating in the outer retina in response to a light stimulus. Defects occurring at various levels of the retina can easily be detected by ERG measurements. Furthermore, the shape of the ERG response points toward the likely retinal cell type responsible for the deficit. Thus, this method is particularly useful for a rapid assessment of retinal function in genetically or pharmacologically manipulated animals. A typical ERG curve can be subdivided into three components: a small initial a-wave originating in photoreceptor activity, a large positive b-wave reflecting mainly ON bipolar cell depolarization, and a d-wave occurring at light offset. Here we present a noninvasive protocol for taking ERG measurements in larval zebrafish (4-7 days post-fertilization [dpf]). We use an extracellular recording electrode which is placed onto the surface of the cornea of the larva, and a light flash of a defined intensity and duration which is applied to evoke a response. In a typical larval ERG trace, we are able to record ERG a-, b-, and d-waves.
引言
视网膜电图（ERG）是一种电生理工具，用于测量外视网膜对光刺激产生的电活动。通过ERG测量可以轻松检测出视网膜不同层面出现的缺陷。此外，ERG反应的形状指向了可能导致缺陷的视网膜细胞类型。因此，该方法对于快速评估基因或药物处理动物的视网膜功能特别有用。典型的ERG曲线可分为三个成分：起源于光感受器活动的小的初始a波、主要反映ON双极细胞去极化的大的正b波以及在光熄灭时出现的d波。在此，我们介绍一种在斑马鱼幼体（受精后4 - 7天[dpf]）进行ERG测量的非侵入性方案。我们使用一个置于幼体角膜表面的细胞外记录电极，并施加具有特定强度和持续时间的闪光来诱发反应。在典型的幼体ERG记录中，我们能够记录到ERG的a波、b波和d波。
