Kiernan John Alan
CSH Protoc. 2008 Jul 1;2008:pdb.top50. doi: 10.1101/pdb.top50.
INTRODUCTIONFor the study of microscopic anatomy and of pathological material, it is usual to stain sections of tissue in such a way as to impart a dark color to the nuclei of cells and a lighter, contrasting color to the cytoplasm and extracellular structures. Nuclear stains, including cationic, anionic, and metal complexing dyes, are considered in this article. The rationales of the techniques are discussed, but methods of higher chemical specificity (e.g., for nucleic acids, carbohydrates, and functional groups of proteins) are not covered.
引言
对于微观解剖学和病理材料的研究，通常以这样一种方式对组织切片进行染色：使细胞核呈现深色，而细胞质和细胞外结构呈现较浅的对比色。本文将探讨包括阳离子、阴离子和金属络合染料在内的细胞核染色剂。文中讨论了这些技术的基本原理，但不涉及具有更高化学特异性的方法（例如针对核酸、碳水化合物和蛋白质官能团的方法）。
