Department of Evolutionary Biology, Theoretical Biology Unit, University of Vienna, Vienna, Austria.
J Anat. 2021 Apr;238(4):1036-1041. doi: 10.1111/joa.13351. Epub 2020 Nov 2.
X-ray microtomography (microCT) enables histological-scale 3D imaging of many types of biological samples, but it has yet to rival traditional histology for differentiation of tissue types and cell components. This report presents prima facie results indicating that a simple lead(II) acetate staining solution can impart preferential X-ray contrast to cell nuclei. While not strictly selective for nuclei, the staining reflects local cell-density differences. It can be applied in a single overnight treatment and does not require hematoxylin staining or drying of the sample. The stain is removable with EDTA, and it may enhance early calcifications. A basic protocol is given as a guide for further testing and optimization.
X 射线显微断层扫描（microCT）能够实现多种类型生物样本的组织学尺度 3D 成像，但在区分组织类型和细胞成分方面，它尚未能与传统的组织学相媲美。本报告初步结果表明，一种简单的醋酸铅（II）染色溶液可以赋予细胞核优先的 X 射线对比。虽然这种染色方法并不严格针对细胞核，但它反映了局部细胞密度差异。该染色方法可以在一个过夜处理中应用，不需要对样本进行苏木精染色或干燥。该染色剂可以用 EDTA 去除，并且可能增强早期钙化。本文提供了一个基本方案作为进一步测试和优化的指南。
