Institute of Physical Chemisty, Friedrich Schiller University, Jena, Germany.
Anal Bioanal Chem. 2011 Jul;400(9):2755-61. doi: 10.1007/s00216-011-4811-z. Epub 2011 Feb 26.
In this contribution, the great potential of surface enhanced Raman spectroscopy (SERS) in a lab-on-a-chip (LOC) device for the detection of analyte molecules in a complex environment is demonstrated. Using LOC-SERS, the enzyme activity of thiopurine S-methyltransferase (TPMT) is analysed and identified in lysed red blood cells. The conversion of 6-mercaptopurine to 6-methylmercaptopurine catalysed by TPMT is observed as it gives evidence for the enzyme activity. Being able to determine the TPMT activity before starting a treatment using 6-mercaptopurine, an optimized dosage can be applied to each patient and serious toxicity appearing within thiopurine treatment will be prevented.
在本研究中，展示了基于微流控芯片的表面增强拉曼光谱（SERS）在复杂环境中分析物分子检测方面的巨大潜力。利用 LOC-SERS，对裂解红细胞中的硫嘌呤 S-甲基转移酶（TPMT）的酶活性进行了分析和鉴定。TPMT 催化 6-巯基嘌呤转化为 6-甲基巯基嘌呤，这为酶活性提供了证据。在使用 6-巯基嘌呤开始治疗之前，能够确定 TPMT 活性，从而可以为每个患者应用优化的剂量，防止巯嘌呤治疗中出现严重的毒性。
