Division of Surgical Oncology, Department of Surgery, James Graham Brown Cancer Center, Louisville, Kentucky, USA.
J Surg Oncol. 2011 Jul 1;104(1):22-8. doi: 10.1002/jso.21899. Epub 2011 Feb 28.
The use of thermal tumor ablative techniques in the pancreas is limited due to the risk of pancreatitis and damage to major vascular structures. Irreversible electroporation (IRE) is a non-thermal ablation technique that could allow ablation in the pancreas while preserving vital surrounding blood vessels. The aim of this study was to assess the safety and ablation volume of IRE in porcine pancreatic tissue.
IRE of swine pancreases was performed using an open technique and ultrasound guidance and the animals were followed for 72 hr, 7 days, and 14 days post-IRE.
All pigs underwent IRE, with one pig was unsuccessful after three attempts using at 3,000 V due to inability to achieve a stable current. All animals recovered well and revealed only mild adhesions, no ascites, and no pancreatic necrosis. All animals had transient increases in amylase and lipase that normalized on post-IRE day 3. Pathologic analysis revealed that ablation defects (median size 3 cm × 2.8 cm) were seen in electroporated areas with significant destruction of the pancreatic tissue with patent vascular structures.
This animal model demonstrates that IRE of the pancreas performed at an optimal voltage is well tolerated, with rapid resolution of pancreatic inflammation and preservation of vascular structures.
由于胰腺炎和大血管结构损伤的风险，热肿瘤消融技术在胰腺中的应用受到限制。不可逆电穿孔（IRE）是一种非热消融技术，它可以在保留周围重要血管的情况下对胰腺进行消融。本研究旨在评估IRE 在猪胰腺组织中的安全性和消融体积。
采用开放式技术和超声引导对猪胰腺进行 IRE，动物在 IRE 后 72 小时、7 天和 14 天进行随访。
所有猪均成功进行了 IRE，其中一只猪在三次尝试使用 3000V 后因无法稳定电流而失败。所有动物均恢复良好，仅出现轻度粘连、无腹水、无胰腺坏死。所有动物的淀粉酶和脂肪酶均短暂升高，在 IRE 后第 3 天恢复正常。病理分析显示，电穿孔区域出现消融缺陷（中位大小为 3cm×2.8cm），胰腺组织明显破坏，血管结构通畅。
该动物模型表明，在最佳电压下进行的胰腺 IRE 耐受良好，胰腺炎症迅速消退，血管结构得以保留。
