Zhang Zhong-yi, Chen Hui, Yang Yan-hui, Chen Ting, Lin Rui-yu, Chen Xin-jian, Lin Wen-xiong
Department of Chinese Medicinal Materials, Henan Agricultural University, Zhengzhou, China.
Ying Yong Sheng Tai Xue Bao. 2010 Nov;21(11):2843-8.
In this paper, T-RFLP (terminal restriction fragment length polymorphism) technique was adopted to study the dynamic changes of bacterial community in the rhizosphere soil of continuously cropped Rehmannia glutinosa L. The results showed that the Shannon diversity index, Margalef index, and similarity index of bacterial community in the rhizosphere soil all decreased in the order of control > one-year cropping > two-year continuous cropping. Under continuous cropping, the proportion of dominant bacterial species declined obviously. In one-year cropping soil, the class Bacilli of phylum Firmicute dominated the bacteria community; while in two-year continuous cropping soil, the class Epsilonproteobacteria of phylum Proteobacteria became dominant. Continuous cropping of R. glutinosa decreased the bacteria species, and simplified the bacterial community structure. The changes of bacterial community diversity under continuous cropping of R. glutinosa led to the disorder of the functions of bacterial community, and thereby, the destruction of the ecological balance in rhizosphere soil, which might be one of reasons causing the obstacles of continuous cropping of R. glutinosa.
本文采用末端限制性片段长度多态性（T-RFLP）技术，研究连作地黄根际土壤细菌群落的动态变化。结果表明，根际土壤细菌群落的香农多样性指数、玛格列夫指数和相似性指数均表现为对照＞一年连作＞两年连作。连作条件下，优势细菌种类比例明显下降。在一年连作土壤中，厚壁菌门芽孢杆菌纲在细菌群落中占主导地位；而在两年连作土壤中，变形菌门ε-变形菌纲成为优势菌群。地黄连作导致细菌种类减少，细菌群落结构简化。地黄连作下细菌群落多样性的变化导致细菌群落功能紊乱，进而破坏根际土壤生态平衡，这可能是导致地黄连作障碍的原因之一。
