Chen Juan, Zhang Xue-Song, Yang Jia-Xue, Jiao Xiao-Lin, Gao Wei-Wei
The Key Laboratory of Bioactive Substances and Resources Utilization of Chinese Herbal Medicine, Ministry of Education, Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 100193, China.
Zhongguo Zhong Yao Za Zhi. 2012 Dec;37(23):3531-5.
To analyze the effects of continuous cropping and soil treatment on rhizosphere fungal community of Panax quinquefolium, and the correlation between rhizosphere fungal community and growth of P. quinquefolium.
Field plot trail of continuous cultivated P. quinquefolium was conducted, meanwhile continuous cropping soil was treated by organic carbon fertilizer plus methylene dithiocyanate and Kingbo respectively. The rate of seedling survival, root disease index and root weight were investigated. Dilution plating was used to analyze the rhizosphere fungal community, multiple comparisons including H, M(a) and J were performed between rhizosphere fungal community and growth of P. quinquefolium.
As to P. quinquefolium planted in continuous soil, the rate of seedling survival, root weight decreased, root disease index increased remarkably (P<0.05). Meanwhile the fungal counts decreased 50% -63% , the diversity index (H') decreased 39%-43%, while the ratio of Penicillium and Aspergillus increased. There was a significant negative correlation between rhizosphere fungal diversity and P. quinquefolium root disease index (r = -0.970, P=0.006). Organic carbon fertilizer plus methylene dithiocyanate treatment could improve the rate of seedling survival and root weight, and could partly increase the rhizosphere fungal diversity.
In continuous cropping soil of P. quinquefolium, the rhizosphere fungal counts and diversity index decreased, meanwhile the fungal community composition changed. Organic carbon fertilizer plus methylene dithiocyanate treatment could improve the growth of continuous cultivated P. quinquefolium.
分析连作及土壤处理对西洋参根际真菌群落的影响，以及根际真菌群落与西洋参生长之间的相关性。
开展西洋参连作田间小区试验，同时分别用有机碳肥加二硫氰基甲烷和金葵宝对连作土壤进行处理。调查西洋参的成活率、根病指数和根重。采用稀释平板法分析根际真菌群落，对根际真菌群落与西洋参生长进行包括H、M(a)和J的多重比较。
对于种植在连作土壤中的西洋参，其成活率、根重降低，根病指数显著升高（P<0.05）。同时真菌数量减少50%-63%，多样性指数（H'）降低39%-43%，而青霉属和曲霉属的比例增加。根际真菌多样性与西洋参根病指数之间存在显著负相关（r = -0.970，P = 0.006）。有机碳肥加二硫氰基甲烷处理可提高西洋参的成活率和根重，并能部分增加根际真菌多样性。
在西洋参连作土壤中，根际真菌数量和多样性指数降低，同时真菌群落组成发生变化。有机碳肥加二硫氰基甲烷处理可促进连作西洋参的生长。
