Liguori G, Di Onofrio V, Gallé F, Lucariello A, Albano L, Catania M R, Guida M
Department of Studies of Institutions and Territorial Systems, University of Naples Parthenope, Italy.
J Prev Med Hyg. 2010 Sep;51(3):121-4.
Candida albicans is the most common fungal pathogen isolated from clinical samples and is also the most common yeast species carried as a commensal by healthy individuals although some non-C. albicans species account for an important number of infections.
To compare nine phenotypic systems for C. albicans identification [API 20C AUX; RapID Yeast Identification panel (RYIP); Vitek2 ID-YST system; chromogenic media, CHRO-Magar, Oxoid Chromogenic Candida Agar (OCCA), Candida ID2, Candida Identification Agar, CandiSelect 4, and Chromalbicans Agar] with multiplex PCR.
PATIENTS/METHODS: A collection of 390 yeast strains was obtained by routine isolation from oral and vaginal swabs. All of the yeasts isolated were tested for germ tube formation, and then submitted to a multiplex PCR protocol tested in previous studies, and to nine phenotypical commercial methods, together with the reference ATCC strains. Comparison was limited to the ability of the tests to identify C. albicans.
253 isolates were provisionally identified as C. albicans by germ tube, and their identities were further confirmed with the multiplex PCR. Sensitivity of phenotypical systems ranged from 81.9% (Vitek2) to 87.7% (Candida ID2 e CHROMagar). For specificity, the highest value was 96.8% for Candida ID2, and the lowest value (75.1%) was for Chromalbicans Agar.
Although with differences in discriminatory power, the methods tested showed overall acceptable levels of sensitivity and specificity respect to the multiplex PCR; therefore, all could be useful for C. albicans identification where molecular differentiation is not available.
白色念珠菌是从临床样本中分离出的最常见真菌病原体，也是健康个体作为共生菌携带的最常见酵母菌种，尽管一些非白色念珠菌菌种也导致了大量感染。
比较用于白色念珠菌鉴定的九种表型系统[API 20C AUX；RapID酵母鉴定板（RYIP）；Vitek2 ID-YST系统；显色培养基，CHRO-Magar、Oxoid显色念珠菌琼脂（OCCA）、念珠菌ID2、念珠菌鉴定琼脂、CandiSelect 4和Chromalbicans琼脂]与多重PCR。
患者/方法：通过常规方法从口腔和阴道拭子中分离出390株酵母菌株。对所有分离出的酵母进行芽管形成测试，然后将其提交至先前研究中测试过的多重PCR方案以及九种表型商业方法，并与参考ATCC菌株一起进行测试。比较仅限于这些测试鉴定白色念珠菌的能力。
253株分离株通过芽管初步鉴定为白色念珠菌，其身份通过多重PCR进一步确认。表型系统的敏感性范围为81.9%（Vitek2）至87.7%（念珠菌ID2和CHROMagar）。对于特异性，念珠菌ID2的最高值为96.8%，Chromalbicans琼脂的最低值为75.1%。
尽管在鉴别能力上存在差异，但所测试的方法相对于多重PCR显示出总体可接受的敏感性和特异性水平；因此，在无法进行分子鉴别时，所有这些方法都可用于白色念珠菌的鉴定。
